摘要
目的在成功建立牙周致病菌皮下感染和腹主动脉瘤复合动物模型的基础上,初步评价牙周致病菌对腹主动脉瘤形成的作用。方法选择6-8周齢雄性C57BL/6小鼠,皮下金属螺旋管植入3周后,CaCl2涂抹法制备腹主动脉瘤模型,每周在螺旋管内注射牙龈卟啉单胞菌构建牙周致病菌感染模型,第2-7d抽取0.1mL螺旋管内液体进行细菌培养。模型制备8周末处死取血和腹主动脉。腹主动脉行HE染色和弹力纤维染色进行病理分析。酶联免疫吸附测定法测量血清中抗牙龈卟啉单胞菌特异性抗体。结果螺旋管腔内1周内均有活菌检出,血清抗牙龈卟啉单胞菌特异性抗体滴度显著高于非感染组。CaCl2处理8周末,牙龈卟啉单胞菌感染组小鼠的腹主动脉扩张率显著大于未感染组（P〈0.01）。病理结果显示CaCl2处理的两组血管结构均有不同程度的破坏,但牙龈卟啉单胞菌感染组小鼠较未感染组腹主动脉弹力纤维破坏更为显著,部分缺失断裂（P〈0.01）。结论本实验成功建立牙周致病菌慢性感染和腹主动脉瘤的动物模型。牙周致病菌感染可能加速实验性腹主动脉瘤的形成和发展。
Objective To investigate the effect of peridontopathogen Porphyromonas gingivalis （Pg） on abdominal aortic aneurysms （AAA） on a murine model. Methods Abdominal aortic aneurysms was established in mice by the periaortic application of CaCl2, and NaCl served as control. The mice were inoculated with live Pg or vehicle once a week. Results Eight weeks after the periaortic application of either CaC12 or NaCl, a significant increase was observed in the aortic diameter in the CaCl2 treatment groups. Furthermore, the change of the aortic diameter was more significant in the combined Pg-challenged mice than that in CaCl2-only mice （ P 〈 0. 05 ）. There was a significant difference in abdominal aortic dilatation between Pg ＋ CaCI： and CaCl2-only treatment groups. Conclusion The animal model was successfully established in this study. Peridontopathogen infection may accelerate the progression of experimental abdominal aortic aneurysms.
出处
《北京口腔医学》
CAS
2016年第1期6-9,共4页
Beijing Journal of Stomatology
基金
国家自然科学基金青年项目(30901678)
北京市科技新星项目(2009B41)
关键词
牙龈卟啉单胞菌
腹主动脉瘤
动物模型
Porphyromonas gingivalis
Abdominal aortic aneurysms
Animal model
