摘要
目的 评价太芪培元颗粒体外对HIV-1 病毒的抑制活性.方法 MTT 法检测太芪培元颗粒细胞毒性;通过细胞病变法检测太芪培元颗粒对HIV-1 急性感染的抑制活性;应用HIV-1 p24 抗原ELISA 检测太芪培元颗粒对HIV-1 急性感染细胞中病毒复制的抑制活性,并计算药物治疗指数.结果 太芪培元颗粒细胞毒性实验CC50 为（3.761±0.370）mg/mL;合胞体抑制实验EC50 为（0.454 5±0.204 6）mg/mL,治疗指数为5.84～12.97;p24 抑制实验EC50 为（0.56±0.27）mg/mL,治疗指数为5.30～8.74.结论 太芪培元颗粒体外抗HIV-1活性较弱.
ObjectiveTo evaluate the vitro anti-HIV-1 activity ofTaiqi Peiyuan Granules.MethodsMTT was used to detect cytotoxicity ofTaiqi Peiyuan Granules; cytopathy method was used to detect the inhibitory activity of Taiqi Peiyuan Granules on acute infection of HIV-1; HIV-1 p24 antigen ELISA detection was used to detect inhibitory activity ofTaiqi Peiyuan Granules for virus replication of HIV-1 acute infection cells, and count the medical therapeutic indexes.ResultsCC50ofTaiqi Peiyuan Granules in cytotoxicity test was 3.761±0.370 mg/mL; the EC50 of inhibition syncytial test was 0.454 5±0.204 6 mg/mL; the therapeutic index was between 5.84 and 12.97; p24 ofTaiqi Peiyuan Granules in inhibition experiments was 0.56±0.27 mg/mL, and the therapeutic index was between 5.30 and 8.74.ConclusionAnti-HIV-1 activity ofTaiqi Peiyuan Granules is relatively weak.
出处
《中国中医药信息杂志》
CAS
CSCD
2016年第4期61-63,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家科技重大专项(2014ZX10005003)
新疆医科大学科研创新基金项目(XJC201181)
关键词
太芪培元颗粒
人类免疫缺陷病毒
体外抗病毒活性
免疫机制
Taiqi Peiyuan Granules
human immunodeficiency virus
vitro antiviral activity
immunologic mechanism
