摘要
[目的]建立火焰原子吸收分光光度法测定丹红注射液中钠（Na）、钾（K）离子含量的方法。[方法]样品经微波消解系统消解后,采用火焰原子吸收分光光度法,以氯化铯为电离抑制剂,分别于589.0 nm和766.9 nm处测定丹红注射液中Na、K离子的含量。[结果]Na离子在0.1～0.6 mg/L范围内线性关系良好,r=0.999 8;平均加样回收率（n=6）101.1%,RSD为1.8%;最低检出限为5.3μg/L。K离子在0.1～0.8 mg/L范围内的线性关系良好,r=0.999 8;平均加样回收率（n=6）99.8%,RSD为2.3%;最低检出限为7.7μg/L。10批丹红注射液样品测定结果表明：Na离子的含量为562.3～734.7 mg/L,K离子含量为206.9～314.4 mg/L。[结论]火焰原子吸收分光光度法操作简单快速,结果准确可靠,重复性好,适用于丹红注射液中Na、K离子的含量测定,可用于丹红注射液质量控制研究。
[Objective] To establish and validate the determination method of the content of sodium ion and potassium ion in Danhong injection by atomic absorption spectrometry（AAS）. [Methods] Microwave digestion system was applied to deteriorate samples, and the content of sodium ion and potassium ion was determined by AAS. [Results] The calibration curve of sodium was liner（r=0.999 8） in the range from 0.1 to 0.6 mg/L. The average recovery was 101.1% with RSD 1.8%. The limit of detection was 5.3 μg/L. The calibration curve of potassium was liner（r =0.999 8） in the range from 0.1 to 0.8 mg/L. The average recovery was 99.8% with RSD 2.3%. The limit of detection was 7.7 μg/L. The determination results of 10 batches Danhong injection sample showed that the content of sodium ion was562.3 to 734.7 mg/L and potassium ion was 206.9 to 314.4 mg/L. [Conclusion] The method is simple and accurate and can be used for determination of the contents of sodium ion and potassium ion in Danhong injection. The accurate quantitative determination method can be promoted to quality control of Danhong injection.
出处
《天津中医药》
CAS
2016年第1期43-46,共4页
Tianjin Journal of Traditional Chinese Medicine
基金
十二五重大新药创制科技重大专项(2013ZX09201020)
国家自然科学基金(81503239)
