摘要
目的探讨微小RNA-338-3p(miR-338-3p)在胃癌组织中的差异表达及其表达与胃癌临床病理特征的关系。方法收集手术切除的51例胃癌标本及配对癌旁胃组织标本,采用实时定量RT-PCR技术检测miR-338-3p在胃癌组织及配对癌旁胃组织中的表达,并探讨其表达与胃癌临床病理参数间的关系。结果与配对癌旁胃组织比较,miR-338-3p在34例胃癌组织中呈低表达,相对表达量为(0.36±0.16),在17例胃癌组织中呈高表达,相对表达量为(1.71±0.66),差异有统计学意义(P<0.05)。miR-338-3p的表达水平与肿瘤浸润深度、淋巴结转移及TNM分期密切相关(P均<0.05),而与年龄、性别及组织学分级无明显相关性(P均>0.05)。结论 miR-338-3p在胃癌组织中低表达,可能参与胃癌的发生、发展,可能成为胃癌诊断及判断病情的新方向。
Objective To investigate the microRNA-338-3p（miR-338-3p）differential expression patterns in gastric carcinoma and adjacent non-tumorous tissues and their relationship with clinicopathological features.Methods 51 pairs of fresh gastric cancer tissues and corresponding normal gastric epithelium were collected.Quantitative real-time PCR was used to detect miR-338-3p expression of all the samples.The relationship between miR-338-3p expression and clinicopathological factors of gastric carcinoma was analyzed.Results 34 patients were with low expression level of miR-338-3p in the 51 patients and 17 patients were with high expression level of miR-338-3p（0.39±0.14 vs 3.08±1.79,P〈0.05）.The expression level was positively correlated to local invasion（P =0.047）,lymph node metastasis（P =0.001）and advanced TNM stage（P =0.017）,but not associated significantly with age,gender and differentiation（P〉0.05）.Conclusion The expression level of miR-338-3p might be low in patients with gastric carcinoma,which could be correlated with the generation and development of diseases.Thus,miR-338-3p can be a new direction in diagnosis and predicting the state of gastric carcinoma.
出处
《新疆医科大学学报》
CAS
2016年第3期308-311,共4页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区自然科学基金(2013211A067)
