摘要
目的探索乳酸杆菌的免疫调节及其抗过敏作用。方法将雄性BALB/C小鼠随机分为空白对照组和干预组(每组24只)。采用灌胃方式,分別连续投用乳酸杆菌植物乳杆菌LP45、嗜酸乳杆菌La28、嗜酸乳杆菌6091、鼠李糖乳杆菌GG共28d。于14d、28d分别用测定免疫脏器系数与血清Th1细胞因子〔干扰素-γ(IFN-γ)、白细胞介素(IL)-12〕、Th2细胞因子(IL-6)含量的方法分析和对比受试菌株的免疫调节作用。对在免疫调节作用上有差异的菌株,利用卵清白蛋白(OVA)和氢氧化铝致敏的BALB/C小鼠过敏模型进一步评价其抗过敏作用。将雄性BALB/C小鼠随机分为空白对照组、OVA阳性对照组和2个菌株干预组,采用ELISA法测定血清总IgE含量。结果供试4个菌株干预组的免疫脏器系数差异无统计学意义;14d、28d时嗜酸乳杆菌La28组血清IL-6含量均低于空白对照组,差异有统计学意义(P<0.05);28d时嗜酸乳杆菌6091组血清IL-6含量低于空白对照组,差异有统计学意义(P<0.05)。在抗过敏作用评价实验中,嗜酸乳杆菌La28组血清总IgE含量高于空白对照组,低于OVA阳性对照组,差异有统计学意义(P<0.05)。结论 4个菌株中嗜酸乳杆菌La28与6091具有免疫调节作用,其中嗜酸乳杆菌La28可能具有抗过敏作用,可能的相关机制为抑制Th2细胞活性,从而影响Th1/Th2平衡。
Objective This study was conducted to evaluate the potent immunomodulatory effects of Lactobacilli and their possible anti-allergic effects.Methods Lactobacillus plantarum LP45（LP45）,Lactobacillus acidophilus La28（La28）,Lactobacillus acidophilus 6091（6091）,Lactobacillus rhamnosus GG（LGG）were orally administrated to male BALB/C mice,respectively for 28 d.The immune organ index,serum Th1cytokines〔interferon-γ（IFN-γ）,interleukin-12（IL-12）〕and Th2 cytokines IL-6of the tested mice were analyzed with ELISA after intervention.Furthermore,La28,6901 were also orally fed to ovalbumin（OVA）-sensitized male BALB/C.The serum total IgE of the tested mice was analyzed with ELISA after intervention.Results No statistical difference was found in immune organ index among the tested four strains.La28 significantly decreased serum IL-6of the tested mice after 14 dand 28dcompared to those in control（P0.05）.After 28 d,6091also significantly reduced serum IL-6of the tested mice（P0.05）.La28 significantly suppressed the increase of serum total IgE of the tested mice（P0.05）.Conclusion The present study indicates that the immunomodulatory effects of Lactobacilli might be strain-dependent.Among the tested strains of Lactobacilli,La28 and 6091 may have possibility to influence the Th2 immunity of host animal.La28 may also posse potent ability to alter IgE mediated allergy by the way to affect Th1/Th2 balance of host animal.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2016年第2期192-196,共5页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金(No.81372982)资助
关键词
乳酸杆菌
免疫调节作用
抗过敏作用
Lactobacilli
Immunomodulatory effect
Anti-allergy effect
