摘要
2015年6月以来,我国部分地区所饲养的肉鸡与蛋鸡发生一种疾病,以心包积液、肝炎为特点,根据病变,暂将该病称为鸡心包积液-肝炎综合征。从不同地方的发病鸡群采集20份病料,发病鸡均能检出禽腺病毒。将阳性病料接种9日龄SPF鸡胚,获得1株禽腺病毒分离株SDSX1。对SDSX1株的hexon基因序列进行的分析表明,SDSX1株与禽腺病毒4型具有相近的遗传进化关系;用其感染1日龄SPF鸡,可复制出心包积液、肝炎的病变。上述结果表明,从鸡心包积液-肝炎自然病例分离到的一种禽腺病毒与鸡心包积液-肝炎综合征有关。
From June 2015,a disease characterized by pericardial effusion and hepatitis was observed in many broiler and egg-laying farm in China. Based on the pathological changes,the disease was tentatively called chicken pericardial effusion and hepatitis syndrome.Twenty samples were collected from the diseased chicken in different areas and were subjected to virus isolation using SPF chicken embryos.One isolate was obtained from livers.PCR results showed that the isolate was positive for fowl adenovirus,designated SDSX1.The isolate was selected for further investigation.Sequence analysis based on a 743 bp hexon gene revealed that strain SDSX1 was most closely related to fowl adenovirus 4(FAV-4).Experimental infections resulted in pericardial effusion andhepatitis.The results suggested that FAV-4 was isolated from the chicken pericardial effusion and hepatitis syndrome(CPEHS)cases,which was caused by FAV-4 infection.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2016年第2期157-160,共4页
Chinese Veterinary Science
基金
河北省现代农业产业技术体系蛋鸡产业创新团队专项资金
关键词
鸡心包积液-肝炎综合征
肉鸡
禽腺病毒4型
chicken pericardial effusion and hepatitis syndrome(CPEHS)
broiler
fowl adenovirus 4