摘要
目的研究芍药苷对小鼠急性炎症肝损伤的保护作用。方法小鼠分为对照组(生理盐水)、模型组(脂多糖,5 mg·kg^(-1))、单用芍药苷组(30 mg·kg^(-1))、脂多糖(5 mg·kg^(-1))+小剂量芍药苷组(10 mg·kg^(-1))、脂多糖(5 mg·kg^(-1))+大剂量芍药苷组(30 mg·kg^(-1)),给药体积为0.2 m L/10 g。腹腔注射给药7 d后建立急性脂多糖炎症模型。酶联免疫吸附法检测肿瘤坏死因子(TNF-α)和白细胞介素1-β(IL-1β)水平;比色法检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、还原型谷胱甘肽(GSH)、一氧化氮(NO)和诱导型一氧化氮合酶(i NOS)水平;蛋白质印迹法检测转录因子(NF-κB)蛋白表达水平。结果芍药苷使小鼠TNF-α、IL-1β、ALT、AST、MDA、NO水平和i NOS活力下降,SOD、CAT、GSH-Px活性和GSH水平升高;IκB磷酸化蛋白表达减少,NF-κB p65的核转移显著抑制。结论芍药苷对小鼠急性炎症肝损伤具有一定的保护作用。
Objective To investigate the protective effect of paeoniflorin on acute liver injury in mice. Methods The mice were randomly divided into 5 groups： control group,model group,paeoniflorin（ Pae） alone group,as well as groups of paeoniflorin and lipopolysaccharide（ LPS）.Animals were injected LPS once daily for 7 d to establish the LPS inflammation model in mice except the control and paeoniflorin group. The activation of tumor necrosis factor（ TNF-α） and interleukin-1β（ IL-1β） in serum was evaluated by Enzyme-linked immunosorbent assays（ ELISA）. Then the alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）, malondialdehyde（ MDA）, superoxide dismutase（ SOD）,catalase（ CAT）,glutathione peroxidase（ GSH-Px）,glutathione（ GSH）,nitricoxide（ NO） and inducible nitricoxide synthase（ i NOS） were detected by the colorimetric method. The levels of nuclear factor-κB（ NF-κB）,IκB-α,p-IκB-α protein in subcellular fractions of hepatic tissue were detected by Western blot. Results Compared with model group,the levels of TNF-α,IL-1β,ALT,AST,MDA and NO,and the activities of i NOS reduced in 30 mg·kg^-1Pae group;while the activities of antioxidant enzyme（ SOD,CAT and GSH-PX）and the level of GSH significantly increased in 30 mg·kg^-1Pae group.Furthermore,Compared with model group,the degradation of IκB-α decreased and the activation of NF-κB was inhibited in 30 mg·kg^-1Pae group. Conclusion The paeoniflorin has the protective effects on acute liver injury.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2016年第5期433-436,共4页
The Chinese Journal of Clinical Pharmacology
关键词
芍药苷
肝损伤
脂多糖
抗炎
抗氧化
Paeoniflorin
liver injury
lipopolysaccharide
anti-inflammatory
anti-oxidative
