摘要
目的:优选文蛤水溶性多糖的水提醇沉工艺,为该有效部位的保健品开发提供实验依据。方法:采用蒽酮-硫酸法测定水溶性多糖的含量,检测波长625 nm。以水溶性多糖转移率为指标,通过单因素试验考察提取溶剂p H及绞碎程度对文蛤水溶性多糖转移率的影响。以水溶性多糖转移率为指标,通过正交试验考察加水量、提取时间、提取次数对文蛤水溶性多糖水提工艺的影响。以水溶性多糖得率及质量分数的综合评分为指标,通过正交试验考察浓缩程度、醇沉浓度、醇沉时间对文蛤水溶性多糖醇沉工艺的影响。结果:文蛤水溶性多糖的最佳水提工艺为加3倍水煎煮3次,每次40 min,水提液浓缩至原料的1/2,加乙醇醇沉至体积分数达80%,醇沉12 h,抽滤,60℃减压干燥。文蛤水溶性多糖得率7.71%,质量分数42.53%。结论:优选的文蛤水溶性多糖水提醇沉工艺稳定可行,可兼顾该有效部位的得率和纯度,为该部位的研究与开发提供参考。
Objective： To optimize extraction technology and alcohol precipitation process of water-soluble polysaccharides form Meretrix meretrix,and provide experimental evidence for health products development of this effective part. Method： Content of water-soluble polysaccharides was determined by anthrone-sulfuric acid method at 625 nm.Water-soluble polysaccharides was islolated by water extracting alcohol precipitating method,taking yield,transfer rate and content of polysaccharides as indexes,process conditions were optimized by orthogonal test and single factor test. Result： Optimum process conditions for water-soluble polysaccharides were as followings：extracted thrice with 3 times the amount of water at 80 ℃,40 min for each time,extract concentrated to half of raw material,added ethanol to volume fraction of 80%,alcohol precipitation for 12 hours,dried at 60 ℃.Under these conditions,average yield of water-soluble polysaccharides was 7.71% and its average content was 42.53%. Conclusion： This optimized technology is stable and feasible with high extracting rate and transfer rate of water-soluble polysaccharides in M.meretrix,it can balance purity and extracting rate of this effective part.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第5期18-21,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家公益性行业专项(201305007,201405017)
国家高新技术研究发展计划(863计划)项目(2013AA093003)
江苏高校优势学科建设项目(PAPD)
关键词
文蛤
总多糖
蒽酮-硫酸法
转移率
Meretrix meretrix
total polysaccharides
anthrone-sulfuric acid method
transfer rate
