摘要
目的研究白头翁汤对外阴阴道念珠菌病(VVC)小鼠Th17细胞及炎性因子的调控作用。方法选取昆明种雌性小鼠72只,随机分为空白对照组、模型组、氟康唑治疗组、(7.5、15.0、22.5)g/kg白头翁汤治疗组。通过对注射雌二醇造成假发情期的小鼠阴道灌注白念珠菌复制出VVC小鼠模型,经过7 d治疗,采用稀释涂布法检测VVC小鼠阴道白念珠菌载荷;Gram染色法观察VVC小鼠阴道分泌物白念珠菌形态;ELISA检测VVC小鼠血清中白细胞介素6(IL-6)、IL-17、IL-21、肿瘤坏死因子α(TNF-α)的水平;免疫组织化学染色法检测阴道组织中的转录因子维甲酸相关孤核受体γt(RORγt)的含量。结果成功建立了VVC小鼠模型;与模型组相比,22.5 g/kg白头翁汤治疗组与氟康唑治疗组的VVC小鼠阴道内白念珠菌载荷数量显著性减少;Gram染色显示,VVC小鼠阴道分泌物中,模型组小鼠含有大量白念珠菌菌丝,7.5 g/kg白头翁汤治疗组小鼠仍有大量较长菌丝,15 g/kg白头翁汤治疗组小鼠虽有菌丝但部分白念珠菌为酵母相,22.5 g/kg白头翁汤治疗组与氟康唑治疗组未见菌丝,只有少量酵母相;经过7 d治疗后,与模型组比较,氟康唑治疗组、(15.0、22.5)g/kg白头翁汤治疗组VVC小鼠血清中IL-6、IL-17、IL-21、TNF-α水平降低;氟康唑治疗组、(15.0、22.5)g/kg白头翁汤治疗组VVC小鼠阴道组织中RORγt水平降低。结论白头翁汤可抑制VVC小鼠白念珠菌增殖并降低炎性细胞因子水平。
Objective To explore the possible regulatory effect of Pulsatilla decoction on Th17 cells and inflammatory cytokines of vulvovaginal candidiasis（ VVC） mice. Methods Seventy-two female Kunming mice were randomly assigned into six groups： a blank control group,a VVC model group,a fluconazole group and three Pulsatilla decoction groups（ dose levels： 22. 5,15. 0 and 7. 5 g/kg,respectively）. The VVC mouse models were established by vaginal inoculation with Candida albicans（ C. albicans） in female mice in pseudoestrus state caused by estradiol injection. After 7-day treatment on VVC mice,the vaginal C. albicans burden was assessed using dilution spread plate method; the vaginal C. albicans morphology was observed by Gram staining method; the levels of interleukin 6（ IL-6）,IL-17,IL-21 and tumor necrosis factor α（ TNF-α）in sera were detected by ELISA. The content of the transcription factor retinoid related orphan receptor gamma t（ RORγt） in vaginal tissues was detected by immunohistochemistry. Results The VVC mouse models were successful y developed. After treatment,the vaginal C. albicans burden of the fluconazole group and 22. 5 g/kg Pulsatil a decoction group dropped significantly compared with that of the VVC model group. Gram staining showed that the VVC mice had lots of C. albicans hyphae in vaginal discharge,that 7. 5 g/kg Pulsatilla decoction group remained the mycelia-phase C. albicans,and that 15. 0 g/kg Pulsatilla decoction group had the majority of yeast-phase C. albicans and a few of mycelia-phase,while no hyphae and only very few of yeast-phase C. albicans were observed in 22. 5 g/kg Pulsatilla decoction group and fluconazole group. After7-day treatment,compared with the model group,the levels of IL-6,IL-17,IL-21 and TNF-α in the sera of the fluconazole group,15. 0 and 22. 5 g/kg Pulsatilla decoction groups were reduced significantly and the levels of RORγt in the vaginal tissues of the fluconazole group,15. 0 and 22. 5 g/kg Pulsatilla decoction groups also decreased significantly. Conclusion Pulsatil a decoction could inhibit the proliferation of vulvovaginal C. albicans and reduces the levels of inflammatory cytokines in VVC mice.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2016年第2期153-157,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81573725)
安徽省高校自然科研项目(KJ2015A030)
安徽中医药大学校级科研项目(2015zr006)
