摘要
目的:探讨不同纯化技术对麦冬总皂苷水提液物理化学性质的影响。方法:采用正交试验优选麦冬总皂苷的水提工艺;采用大孔树脂吸附及膜分离等不同技术对麦冬皂苷水提液进行纯化,并测定其电导率、p H、黏度、浊度等理化参数及蛋白质、鞣质、多糖、总皂苷等物质的含量。结果:麦冬总皂苷的最佳水提工艺条件为:每次加6倍量水,煎煮3次,每次90 min;麦冬总皂苷不同纯化液的电导率、p H、黏度无显著影响,但总皂苷及3种大分子物质含量有显著差异。结论:大分子物质的含量可作为考察麦冬总皂苷水提液纯化工艺的参考指标;膜分离技术比大孔树脂吸附更适用于麦冬总皂苷水提液的分离纯化。
Objective： To explore the effect of different separation and purification technology on the physical and chemical properties of water extract of ophiopogonin. Methods： The water extraction process of total ophiopogon saponins was optimized by an orthogonal test. The macroporous resin adsorption and membrane separation technology were adopted to purify the ophiopogonin water extract.The physical and chemical parameters,such as electrical conductivity,p H value,viscosity and turbidity,and the contents of total saponins,proteins,tannins and polysaccharides were determined. Results： The optimum water extraction technology of total saponins was as follows： 6-fold amount of water was added,boiling 3 times with 90 min for each time. The electrical conductivity,p H value and viscosity of different purified liquid of total saponins had no significant differences,while the contents of total saponins and the three kinds of macromolecules showed significant differences. Conclusion： The content of macromolecules can be used as the reference index of purification process of total saponins water extract. Compared with macroporous resin separation,membrane separation technology is more suitable for the separation and purification of total saponins water extract.
出处
《中国药师》
CAS
2016年第3期430-433,共4页
China Pharmacist
基金
国家自然科学基金项目(编号:81373978)
陕西省科技资源开放共享平台项目(编号:2015FWPT-01)
陕西省教育厅项目(编号:320104-203010009)
关键词
麦冬皂苷
水提液
物理化学性质
纯化技术
Ophiopogonin
Water extract
Physical and chemical properties
Purification technology
