摘要
茶树品种真实性鉴定是茶树种质资源研究的重要组成部分,是茶树品种知识产权保护的前提.在茶树基因组上按照均匀分布的原则选择45 个SSR 标记合成荧光标记引物,利用这些标记引物对54 个福建无性系茶树品种进行PCR 扩增,标记基因型分型后进一步筛选出9 个SSR 标记核心引物.这批核心引物的多态性信息含量（Polymorphism information content,PIC）均大于0.5,等位位点数在3-5 之间,基因型数在5-10 之间,属于高度多态性引物且方便进行基因型数据统计,适宜用来进行无性系茶树品种的真实性鉴定.利用其中的6 个标记核心引物建立了54 个福建无性系茶树品种的品种鉴别图（Cultivar identification diagram,CID）,该CID 能直观地提供对其中任意品种进行鉴定所需要的标记引物以及相应的标记基因型,且能够方便地进行扩容以用来对更多的无性系茶树品种进行真实性鉴定.利用该方法对无性系茶树品种进行真实性鉴定,操作方便、快速准确、稳定性高、实用性强.本实验所获得的茶树品种鉴定图（CID）对茶树品种知识产权保护,以及促进茶树品种选育工作健康可持续发展具有重要的意义.
The authenticity identification of tea cultivars is an important part of the research on tea germplasmresources, and the precondition of intellectual property protection of tea cultivars, 45 SSR markers were selected tosynthesize fluorescent labeled SSR primer pairs based on the principle of uniform distribution in the genome of thetea plant. Using these markers, 54 clonal tea cultivars in FUJIAN province were amplified by PCR and 9 SSRmarkers were selected as the core primer pairs after genotyping. The polymorphism information content （PIC） of thecore primer pairs was more than 0.5, and the number of all alleles was varied from 3 to 5, the number of genotypeswas varied from 5 to 10. These core primer pairs were suitable for identifying the authenticity of tea cultivars for 54Fujian clonal tea cultivars due to high polymorphism and convenient to genotype. The cultivar identification diagram（CID） was then established using 6 of these core primer pairs. The CID provided primer pairs and its correspondinggenotype, by which any cultivar in the CID could be identified. The capacity of the CID can be expanded so as to facilitate the authenticity identification of more clonal tea cultivars. The method was considered to be easy, fast, accurate, stable and practical for the authenticity identification of tea cultivars. The CID is important for the protection of intellectual property of tea cultivars, and will be helpful for the healthy and sustainable development of tea breeding.
出处
《茶叶科学》
CAS
CSCD
北大核心
2016年第2期210-218,共9页
Journal of Tea Science
基金
福建省自然科学基金(2015J01098)
福建省省属公益类科研院所基本科研专项(2014R1012-6)
福建省农业科学院茶叶科技创新团队项目(CXTD-1-1302)
安徽农业大学茶树生物学与资源利用国家重点实验室开放基金(LTBB20140102)
关键词
茶树
SSR
品种鉴别图(CID)
品种鉴定
tea plant [Camellia sinensis （L.） O. Kuntze], SSR, cultivar identification diagram（CID）, cultivar identification
