摘要
目的:通过PTU复制大鼠甲状腺肿模型,观察富碘中药复方瘿宁合剂对甲状腺肿大鼠的恢复情况影响。方法:选取4周龄SD大鼠62只,随机选取10只作为正常组,其余52只予以丙硫氧嘧啶(PTU)1 mg/100 g灌胃14 d复制甲状腺肿大鼠模型,随机选取2只大鼠进行模型验证。造模成功后,将50只大鼠按随机对照表随机分为5组,分别为模型对照组、瘿宁合剂1号组、瘿宁合剂2号组、富碘单味药组、碘剂组。瘿宁合剂1号组,予以瘿宁合剂1号浓缩煎剂3 m L灌胃;瘿宁合剂2号组,予以瘿宁合剂2号浓缩煎剂3 m L灌胃;富碘单味药组,予以海藻、海带、昆布浓缩煎剂3 m L灌胃;碘剂组予以碘酸钾溶液3 m L灌胃,以上各组碘含量均为1990μg/m L;模型组予以生理盐水灌胃。记录实验过程中大鼠的体重变化情况,干预28 d后处死大鼠,称取大鼠甲状腺组织重量,进行HE染色观察组织形态。结果:PTU灌胃14 d后,肉眼观察大鼠甲状腺可见其充血肿大,镜下观察可见甲状腺肿形成,甲状腺肿大鼠模型复制成功。与正常组比较,对照组大鼠体重在整个实验过程中呈缓慢增长的趋势,P<0.05;与正常组比较瘿宁合剂1号组、瘿宁合剂2号组、富碘单味药组以及碘剂组体重均呈缓慢增长至增长速度正常的趋势,均P<0.05,各组间比较无统计学差异,P>0.05;镜下观察甲状腺滤泡细胞,富碘中药复方组恢复程度明显好于其他组。结论:PTU能够导致大鼠甲状腺肿大,瘿宁合剂能够使PTU致大鼠甲状腺肿甲状腺滤泡形态得到改善,治疗安全有效。
Objective：To explore the use of iodine-rich herbal decoction Yingning Mixture to treat PTU-induced thyroid goiter in rats recovery. Methods：62 SD rats were randomly selected 10 of the normal group,the remaining 52 rats use propylthiouracil（PTU）1 mg/100 g gavaged for 14 days,two rats were randomly selected model validation. After the modeling,the 50 rats were randomly divided into 5 groups table,model control group,Yingning Mixture group 1,Yingning Mixture group 2,iodine-rich herbal group,iodine group. Yingning Mixture group 1,to be Yingning Mixture group 1 3 m L concentrated decoction gavage;Yingning Mixture group 2,to be Yingning Mixture 2 3 m L concentrated decoction gavage;iodinerich herbal group,to be algae,seaweed,kelp concentrated decoction 3 m L gavage;iodine group use 3 m L of potassium iodate gavage,iodine content of each group were 1990 μg/m L;model group received normal saline. The rats were sacrificed 28 days after the intervention,rats were weighed and the weight of the thyroid tissue,changes in body weight carried HE staining morphology and recorded throughout the experiment rats. Results：PTU gavage for 14 days,rats were observed visually goiter,goiter formation were observed in rats,rats goiter copied successfully. Compared with the normal group,the body weight of rats in the control group showed slow growth trend,P〈0.05;compared with normal group,Yingning Mixture group 1,YingningMixture group 2,iodine-rich herbal decoction group,as well as iodine group showed a weight trend from normal growth rate to slow growth rate,P〈0.05,there was no statistically significant difference between the groups,P〈0.05;thyroid follicular cells were observed,iodine-rich herbal decoction group to restore better than the other groups. Conclusion：PTU rats can cause goiter,an iodine-rich herbal decoction can improve PTU-induced thyroid follicular morphology in rats,treatment is effective.
出处
《辽宁中医药大学学报》
CAS
2016年第3期23-26,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
辽宁省教育厅攻关计划基金资助项目(202153326)
辽宁省科技厅博士基金资助项目(20031039)
