摘要
目的探讨磷酸酶Inpp5f在血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞肥大反应中的作用。方法用AngⅡ(1×10^(-7)mol/L)诱导大鼠心肌细胞H9c2发生肥大反应,qRT-PCR和Western blot分别检测Myh7、Myh6和Inpp5f的表达变化;构建Inpp5f过表达载体,给予细胞过表达外源性Inpp5f后,在AngⅡ诱导的心肌肥大反应中通过qRTPCR和Western blot查看Myh7、Myh6的表达变化。结果在心肌细胞肥大反应中,Inpp5f表达降低。与对照相比,外源性过表达Inpp5f能显著减少AngⅡ引起的Myh7表达升高和Myh6表达降低。结论在心肌肥大反应中,Inpp5f表达降低,而增加Inpp5f的表达能抑制心肌肥大反应,提示其作为心肌肥大保护因子的可能。
AIM To investigate the effect of inositol polyphosphate-5-phosphatase F （Inpp5f） on angiotensin II-induced cardiomyocyte hypertrophy. METHODS Cardiomyocyte hypertrophy was induced by angiotensin II （ 1 × 10 -7 mol/L） in H9c2 cells. Expressions of Myh7, Myh6 and Inpp5f were detected by Western blot and real-time PCR. An Inpp5f overexpression vector was successfully constructed and transfected into H9c2 cells. Transcripts for Myh6 and Myh7 were detected by real-time PCR. RESULTS mRNA and protein levels of Inpp5f significantly decreased in the hypertrophy group. Overexpression of InppSf reversed the angiotensin II-induced increase in mRNA expression level of Myh7 and decrease in mRNA expression level of Myh6. CONCLUSION Overexpression of InppSf attenuates angiotensin II-induced hypertrophy of H9c2 cardiomyocytes.
出处
《心脏杂志》
CAS
2016年第2期145-148,共4页
Chinese Heart Journal
基金
国家自然科学基金项目资助(81101050)
陕西省自然科学基础研究项目资助(2014JM4182)
关键词
磷酸酶
Inpp5f
心肌肥大
血管紧张素Ⅱ
phosphatase
inositol polyphosphate-5-phosphatase F
myocardial hypertrophy
angiotensin II
