摘要
目的:研究CD4^+CD25^+调节性T细胞(regulatory T cells,Tregs)对乳腺癌细胞上皮间质转化(epithelial-mesenchymal transition,EMT)、细胞迁移侵袭能力,及ALDH1^+干样细胞比例的影响。方法:采用免疫磁珠法分离乳腺癌患者外周血中CD4^+CD25^+Tregs,CD4^+CD25^+Tregs与乳腺癌BT474、MCF-7细胞系共培养(共培养组),BT474、MCF-7单独培养(对照组)。检测共培养组和对照组乳腺癌细胞EMT相关标志物表达的变化,及细胞迁移和侵袭能力的变化。此外,检测BT474细胞中ALDH1^+干样细胞、微球形成能力和自我更新能力的变化。结果:CD4^+CD25^+Tregs诱导BT474和MCF-7细胞间质性标志物表达增高,诱导MCF-7细胞上皮性标志物E-cadherin表达降低。CD4^+CD25^+Tregs诱导BT474和MCF-7细胞迁移和侵袭能力上调。共培养组BT474细胞中ALDH1^+干样细胞比例、微球体形成能力、自我更新能力较对照组增强。结论:CD4^+CD25^+Tregs可诱导乳腺癌细胞发生EMT,增强细胞体外迁移和侵袭能力,同时促进ALDH1^+干样细胞增加。
Objective： To investigate the effects of CD4＋CD25＋ regulatory T cells （Tregs） on the epithelial-mesenchymal transition （EMT）, migratory and invasive capacities, and proportion of ALDH1＋ cancer stern cell （CSC）-Iike cells in breast cancer cells. Methods： CD4＋CD25＋ Tregs were isolated from the peripheral blood of breast cancer patients through immunomagnetic sand method. In the co-culture groups, breast cancer cell lines BT474 and MCF-7 were co-cultured with CD4＋CD25＋Tregs. In the control groups, BT474 and MCF-7 cells were cultured alone. EMT-related markers of breast cancer cells were then assayed in the control and co-culture groups. The migrato- ry and invasive capacities of breast cancer cells were also detected in both groups. Furthermore, the changes in ALDHI＋CSC-like cells, mammosphere-forming ability, and se＋f-renewal capacity of BT474 cells were assayed. Results： After co-culture with CD4＋CD2S＋Tregs, the expression of mesenchymal biomarkers increased in BT474 and MCF-7 cells, whereas the expression of the epithelial biomarker E- cadherin decreased in MCF-7 cells. Additionally, the migratory and invasive capacities of BT474 and MCF-7 cells increased after co-cul- ture. Overall, the subpopulation of ALDH1＋CSC-iike cells, the mammosphere-forming ability, and the self-renewal capacity of BT474 cells improved in the co-culture group compared with the control group. Conclusion： The EMT process, migratory and invasive capaci- ties, and CSC-like properties of breast cancer cells can be enhanced by CD4＋CD25＋regs.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2016年第5期177-182,共6页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金项目(编号:81171983)资助~~
关键词
调节性T细胞乳腺癌上皮间质转化ALDH1+干样细胞
regulatory T cells, breast cancer, epithelial-mesenchymal transition, ALDH1＋cancer stem cell-like cells
