摘要
以反丁烯二酸和氨水为原料,采用天冬氨酸酶基因工程菌固定化细胞生物催化法合成L-天冬氨酸。通过响应面法考察反丁烯二酸浓度、温度、p H对合成L-天冬氨酸的影响。结果表明,固定化天冬氨酸酶基因工程菌合成L-天冬氨酸最佳条件为:底物反丁烯二酸的质量浓度为300 g/L,反应温度为37℃,底物p H为7.5,L-天冬氨酸的产率为96.7%。固定化细胞可连续使用10批次。通过电镜观察发现天冬氨酸酶基因工程菌均匀分布于载体,天冬氨酸酶基因工程菌固定化细胞具有良好的稳定性。
With fumaric acid and ammonium hydroxide as raw materials,synthesis of L-aspartic acid by immobilized cells of aspartase engineering bacteria was carried out. The effect of fumaric acid concentration,reaction temperature and p H was investigated by response surface method. The results show that the optimized preparation conditions were as follows： 300 g / L fumaric acid,37 ℃,p H = 7. 5,the yield of L-aspartic acid was 96. 7%. The immobilized bacteria could be used continuously for 10 times. The aspartase engineering bacterias were distributed evenly over the carrier by electron microscope. The immobilized cells of aspartase engineering bacterias have good stability.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2016年第3期302-306,共5页
Fine Chemicals
基金
安徽省优秀青年人才基金重点项目(2013SQRL086ZD)
宿州学院教授(博士)科研启动基金项目(2014jb06)
安徽省质量工程项目(2015zjjh034
2015ckjh108)
宿州区域发展协同创新中心开放课题(2015SZXTZXKFYB01)~~
关键词
L-天冬氨酸
固定化
基因工程菌
生物工程
L-aspartic acid
immobilized
engineering bacteria
biological engineering
