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烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1信号肽预测及分析 被引量：5

Analysis of coding region for proteins containing signal peptides of Pseudomonas syringae pv. tabaci yuexi-1 strain
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摘要 利用Signal P 4.0、Lipo P 1.0及TMHMM v2.0对烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1菌株基因组中信号肽的数量、长度和氨基酸组成进行了预测及分类。结果确定其中432个ORFs（Open reading frame）所编码的N端有信号肽序列,占全部ORFs的8.81%。其中351条分泌型信号肽（SPI）,81条脂蛋白型信号肽（SPII）。在分泌型信号肽中,信号肽的长度为11~42个氨基酸,以长度为22个氨基酸的信号肽最多。同源性分析结果显示,具有相同信号肽序列的不同蛋白序列之间是高度保守的。该研究提供了野火病原菌致病因子的备选基因,提高该病菌致病因子的筛选效率。 This paper predicted and analyzed the number, length and amino acid components in the genome of Pseudomonas syringae pv. tabaci yuexi-1, a strain of tobacco fire blight pathogen, by using Signal P 4.0, Lipo P 1.0 and TMHMM v2.0. Results showed that 432 ORFs（8.81% of all the ORFs） contained N- terminal signal peptides, of which 351 signal peptides were SPI type and 81 signal peptides were SPII type. Among the SPI peptides, length was between 11 to 42 amino acids and the majority was 22 amino acids in length. In addition, the proteins that share the same signal peptide sequences were highly conserved in the homologue analysis. This study provided candidate genes for virulence factors of the tobacco fire blight pathogen, and promoted the efficiency for screening the virulence factors.
作者 王铁霖 李晶 杨玉文 赵廷昌
机构地区 中国农业科学院植物保护研究所
出处 《中国烟草学报》 EI CAS CSCD 北大核心 2016年第1期92-100,共9页 Acta Tabacaria Sinica
基金 烟草病虫害检测与综合治理重点开放实验室开放课题经费(项目号:bc2011) 中国烟草总公司科技重点项目(项目号110201202002)
关键词 Pseudomonas SYRINGAE pv.tabaci 信号肽 Signal P 4.0 Lipo P 1.0 TMHMM v2.0 Pseudomonas syringae pv tabaci signal peptide Signal P 4 0 Lipo P 1 0 TMHMM v2 0
分类号 S435.72 [农业科学—农业昆虫与害虫防治]
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参考文献25

  • 1Gasson M J.Indicator technique for antimetabolic toxin production by phytopathogenic species of Pseudomonas[J].Applied and environmental microbiology,1980,39(1):25-29.
  • 2Thomas M D,Uchytil TF,Durbin RD.et al.Inhibition of glutamine synthetase from pea by tabtoxinine-beta-lactam[J].Plant physiology,1983,71(4):912-5.
  • 3Emanuelsson O,von Heijne G.Prediction of organellar targeting signals[J].Biochimica et Biophysica Acta(BBA)-Molecular Cell Research,2001,1541(1):114-119.
  • 4Baldi P,Brunak S,Chauvin Y,et al.Assessing the accuracy of prediction algorithms for classification:an overview[J].Bioinformatics,2000,16(5):412-424.
  • 5Heijne G.Patterns of amino acids near signal‐sequence cleavage sites[J].European journal of biochemistry,1983,133(1):17-21.
  • 6Von Heijne G.Signal sequences:the limits of variation[J].Journal of molecular biology,1985,184(1):99-105.
  • 7王铁霖,严婉荣,闫莎莎,杨玉文,赵廷昌.瓜类果斑病菌(Acidovorax citrulli)基因组信号肽预测分析[J].中国瓜菜,2012,25(1):1-6. 被引量：2
  • 8Tjalsma H,van den Dolder J,Meijer WJ,et al.The Plasmid-Encoded Signal Peptidase Sip P Can Functionally Replace the Major Signal Peptidases Sip S and Sip T of Bacillus subtilis[J].Journal of bacteriology,1999,181(8):2448-2454.
  • 9Tjalsma H,Zanen G,Venema G,et al.The potential active site of the lipoprotein-specific(type II)signal peptidase of Bacillus subtilis[J].Journal of Biological Chemistry,1999,274(40):28191-28197.
  • 10Prágai Z,Tjalsma H,Bolhuis A,et al.The signal peptidase II(Isp)gene of Bacillus subtilis[J].Microbiology,1997,143(4):1327-1333.

二级参考文献19

  • 1刘雅婷,李正跃,朱有勇,李成云,李永忠.植物病原细菌Pseudomonas syringae pv.tomato基因组中的信号肽分析[J].遗传,2005,27(6):959-964. 被引量：12
  • 2Tjalsma H,Bolhuis A ,van RooSmalen M L,et al. Functional analysis of the secretory precursor processing machinery of Bacillus subtilis : identification of a eubacterial homolog of archaeal and eukaryotic signal peptidases [J]. Genes & Development, 1998,12 ( 15 ) : 2318-2331.
  • 3Tjalsma H,Kontinen V P,Pragai Z,et al. The role of lipoprotein processing by signal peptidase Ⅱ in the Gram-positive eubacterium Bacillus subtilis : signal pep tidases Ⅱ is required for the efficient secretion of a-amylase. Anon-lipop retein[J]. Journal of Biology Chemistry, 1999,274(3) : 1698-1707.
  • 4Tjalsma H,Zanen G, Venema G,et al. The potential active site of the , lipoprotein-specifie ( type Ⅱ ) signal peptidase of Bacillus subtilis [J]. Jouma of Biology Chemistry, 1999,274(40) : 28191-28197.
  • 5Chang Y S,Dubnau D. Corn C is required for the processing and translocation of ComGC, a pilin-like competence protein of Bacillus subtilis[J]. Molecular Microbiology, 1995,15 (3) : 543-551.
  • 6Paik S H, Chakicherla A, Hansen J N. Identification and characteri- zation of the structural and transporter genes for, and the chemical and biological properties of,sublanein 168,anovel lantibiotie pro- dueed by Bacillus subtilis 168[J]. Journal of Biology Chemistry, 1998, 273(36): 23134-23142.
  • 7Tjalama H, Albert B, Jan D H, et al. Signal peptide-dependent protein transport in Bacillus subtilis :a genome-based survey of the secre-tome[J]. Microbiology and Molecular Biology Reviews ,2000, 64(3) : 515-547.
  • 8Nielsen H, Engelbrecht J, Brunak S, et al. Identification of prokary- otic and eukaryotic signal peptides and prediction of their cleavage sites[ J ]. Protein Engineering, 1997,10( 1 ) : 1-6.
  • 9Juncker A S,Willenbrock H, Von Heijne G,et al. Prediction of lipop rotein signal peptides in Gram-negative bacteria [J]. Protein Science, 2003,12(8) : 1652-1662.
  • 10Nielsen H, Engelbrecht J, Brunak S,et al. Identification of prokary- otic signal peptides and prediction of their cleavage sites[J]. Protein Eng, 1997,10: 1-6.

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中国烟草学报
2016年 第1期

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