摘要
目的研究星形细胞上调基因1(AEG1)和钙黏附蛋白E(E-cad)在食管癌中的表达的相关性,并分析与临床病理特征的关系。方法采用免疫组织化学方法和Western blot两种方法检测58例食管癌组织及其相应的癌旁组织中AEG1和钙黏附蛋白E的表达,并分析与临床病理特征的相关性。结果在58例食管癌组织中AEG1表达的阳性率82.7%明显高于相应的癌旁组织的51.7%(P<0.05),且表达阳性率与性别、年龄、肿瘤的部位、肿瘤病理类型无相关性(P>0.05),而与肿瘤分化程度、浸润深度、临床分期、淋巴结转移相关(P<0.05);在58例食管癌组织中钙黏附蛋白E的阳性率为31%,明显低于相应癌旁组织的94.8%(P<0.05),且表达阳性率与性别、年龄、肿瘤的部位、肿瘤病理类型无相关性(P>0.05),而与肿瘤分化程度、浸润深度、临床分期、淋巴结转移相关(P<0.05);食管癌中AEG1与钙黏附蛋白E的表达呈明显的负相关(r=-0.483,P<0.05)。结论 AEG1和钙黏附蛋白E在食管癌中的表达分别表现为高表达和表达缺失,且两者与食管癌的侵润、转移密切相关,AEG1可能介导食管癌中钙黏附蛋白E的表达下调。
Objective To explore the correlationship of astrocyte elevated gene 1（AEG1） and expression of E-cadherin（E-cad） protein in esophageal carcinoma,and analyze its relationship with clinical pathological features.Methods Immunohistochemical and Western Blot test methods were used to detect the expression of AEG1 and Ecadherin protein in 58 cases of esophageal carcinoma tissues and its corresponding adjacent tissues. Then we analyzed the relationship of AEG1,E-cadherin and clinicopathologic correlation. Results In 58 cases of esophageal carcinoma tissues,the positive expression rate of AEG1 was 82.7 %; The positive expression rate of AEG1 was 51.7% in the corresponding adjacent tissues,the positive expression rate of AEG1 in esophageal carcinoma tissues was obviously higher than that in the corresponding adjacent tissues（P〉0.05）,and the expression positive rate of AEG1 in esophageal carcinoma tissues was related to differentiated degree,depth of invasion,clinical stage,and lymph node metastasis（P〈0.05）; but no relation with sex,age,tumor site,and pathologic types（P〈0.05）; in 58 cases of esophageal carcinoma tissues,the positive expression rate of E-cadherin protein was 31%,the positive expression rate of E-cadherin was 94.8%; the positive expression rate of E-cadherin in esophageal carcinoma tissues was obviously less than that in the corresponding adjacent tissues（P〈0.05）; It is related to differentiated degree,depth of invasion,clinical stage,and lymph node metastasis（P〈0.05）; but no relation with sex,age,tumor site,and pathologic types（P〉0.05）; the expression of AEG1 and E-cadherin protein was significantly negative correlation（r = 0.483,P〈0.05）.Conclusions The expression of AEG1 and E-cadherin in esophageal carcinoma was characterized by over-expression and loss-expression,and they were closely associated with invasion and metastasis of esophageal carcinoma,AEG1 may be mediated E-cadherin less-expression in esophageal carcinoma.
出处
《中国现代医学杂志》
CAS
北大核心
2016年第4期33-38,共6页
China Journal of Modern Medicine
