摘要
Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA （rRNA） gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that ob- taining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infec- tions. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.
芳香黄杆菌广泛耐药,一旦感染很难治愈。其耐药机制尚不清楚,需要进一步研究。本文对国内外的芳香黄杆菌病例进行全面总结,分析该细菌的抗生素耐药情况、耐药机制、病人预后、医院内爆发感染和流行的风险,以及目前实验室应用的诊断方法,指出全基因组测序应用于阐明其耐药机制的可行性和迫切性。
基金
Project supported by the Huaqiao University Graduate Student Scientific Research Innovation Ability Cultivation Plan Projects
the Major Program of Department of Science and Technology of Fujian Province(No.2012Y4009)
the Science and Technology Planning Project of Xiamen(No.3502Z20123036)
the Xiamen Southern Oceanographic Center(No.14GYY008NF08)
the Construction Project for Yun Leung Laboratory for Molecular Diagnostics(No.14X30127)
the Technology Planning Projects of Quanzhou Social Development Fields(No.2014Z24)
the Major Support Research Project of National Key Colleges Construction of Quanzhou Medical College(No.2013A13),China
