摘要
目的了解CTX-M-1亚群、CTX-M-9亚群与不产CTX-M3类鸡源大肠杆菌毒力基因的流行特征。方法从河南省不同地区分离获得CTX-M-1亚群鸡大肠杆菌33株,CTX-M-9亚群鸡大肠杆菌31株和不产CTX-M的鸡大肠杆菌23株,以多重PCR技术检测受试菌携带33种毒力基因的情况。结果 3类受试菌毒力基因的携带种类趋于一致,其中feoB、fimH、traT和sitA在3类菌中检出率均较高。CTX-M-1亚群鸡大肠杆菌中traT和irp-2基因和CTX-M-9亚群鸡大肠杆菌中iroN、iss和irp-2基因的携带著高于不产CTX-M菌株。CTX-M-1亚群鸡大肠杆菌中cvaC和sfaS的携带率高于CTX-M-9亚群,而iroN和iss的携带率低于CTX-M-9亚群。结论结果表明CTX-M-1与CTX-M-9亚群大肠杆菌部分毒力基因的携带率明显高于不产CTX-M的菌株,CTX-M-9和CTX-M-1亚群鸡大肠杆菌携带的部分毒力基因差异明显,推测产CTX-M鸡大肠杆菌的致病力可能较强,且菌株的致病力可能还与其携带的CTX-M基因亚型有关。
This research was conducted to explore the epidemiologies of the virulence genes in CTX-M-1cluster Escherichia coli,CTX-M-9cluster isolates and non-CTX-M ones by multiplex-PCR.The strains,including 33CTX-M-1 Escherichia coli isolates,31CTX-M-9isolatesand 23non-CTX-M ones,were isolated from chickens in Henan Province.The results showed that all of E.coli contained almost similar virulence genes and the detection rates of feoB,fimH,traT,and sitA were higher than the others.The CTX-M-1cluster and the CTX-M-9cluster E.coli harbored significantly more virulence factors than nonCTX-M isolates,with the traT and irp-2in the CTX-M-1cluster and the iron,iss and irp-2in CTX-M-9cluster.Moreover,the partial virulence factors showed significantly difference between the CTX-M-1cluster and the CTX-M-9cluster.The cvaC and sfaS genes in the former were significantly more than those in the latter,but the iroN and iss genes were significantly fewer.In conclusion,the pathogenicities of CTX-M-producing E.coli maybe more powerful,and the CTX-M genotypes maybe had close relationship with their pathogenicity.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2016年第2期152-155,共4页
Chinese Journal of Zoonoses
基金
国家自然科学基金项目(No.31201965)资助~~
