摘要
目的研究大黄素对肾毒血清肾炎大鼠核因子-κB及白细胞介素-1的影响。方法肾毒血清肾炎应用兔抗鼠肾小球基底膜肾毒血清制备。实验分组为大黄素组200 mg/(kg·d),肾炎未治疗组及正常对照组。大鼠注射肾毒血清后6 h内开始灌胃给药,对照组仅给等量水。定期观察一般情况,并测定尿蛋白水平,4周后处死大鼠,观察肾组织病理改变。采用流式细胞仪技术检测核因子-κB;采用ELISA检测白细胞介素-1(IL-1)。结果大鼠注射抗血清后24 h内即出现大量尿蛋白,其后持续维持在较高水平,大鼠肾炎模型建立成功。肾炎未用药组核因子-κB及白细胞介素-1分泌水平增加,与对照组相比差异显著,P<0.05。大黄素组核因子-κB及白细胞介素-1分泌水平下降,与肾炎未用药组相比差异显著,P<0.05;但仍高于对照组,差异无统计学意义,P>0.05。结论大黄素能够降低核因子-κB及白细胞介素-1分泌,改善肾毒血清肾炎大鼠的肾脏病变。
Objective To study the effect of Dahuangsu on the immunizing factor in experimental anti-GBM nephritis rat. Methods Nephrotoxic serum nephritis was induced by injection of anti-GBM antibody into the tail veins of rats. The level of nuclear factor-κB was detected by flow cytometry and IL-1（ IL-1） was detected by ELISA. Results In the nephritic group,cytokines of nuclear factor-κB and interleukin-1（ IL-1） enhanced compared with the control group,with a significant difference,P〈0. 05. In Dahuangsu group,cytokines of nuclear factor-κB and interleukin-1（ IL-1） decreased,there was a significant difference compared with the nephritic group,P〈0. 05. Conclusion Dahuangsu can decrease cytokines of nuclear factor-κB and interleukin-1（ IL-1） in experimental anti-GBM nephritis rat.
出处
《哈尔滨医科大学学报》
CAS
2016年第1期36-38,43,共4页
Journal of Harbin Medical University
关键词
大黄素
肾毒血清肾炎
免疫因子
Dahuangsu
anti-GBM nephritis
immunizing factor
