摘要
目的研究海藻酸钙海绵的止血作用并评价其体外安全性。方法采用Lee-W hite凝血法测定海藻酸钙的最佳凝血浓度,冷冻干燥法制备成海绵,采用体外凝血指数(BCI)法测定海藻酸钙海绵的体外凝血效果,应用新西兰兔耳动脉、背部全皮层止血模型验证海藻酸钙海绵的止血作用,采用MTT法初步评价海藻酸钙海绵的细胞毒性。结果海藻酸钙的体外凝血指数(BCI)值为33.08±4.02,明显低于明胶海绵(72.05±10.48),差异有统计学意义(P<0.05)。在耳动脉模型中,海藻酸钙海绵组的出血量为2.85±0.14g,明显少于医用纱布组(3.93±0.18g,P<0.05)和明胶海绵组(2.97±0.17g,P<0.05);海藻酸钙海绵组止血时间(97.83±5.49s)明显低于医用纱布组(101.00±14.71s,P<0.05),但与明胶海绵组(80.25±7.41s)比较差异无统计学意义(P>0.05)。在背部全皮层止血实验中,海藻酸钙海绵组的出血量吸光度(A)值为2.66±0.24,明显低于医用纱布组(3.81±0.73,P<0.05)。细胞毒性实验结果显示,100%浓度的海藻酸钙海绵浸提液细胞毒性为1级,属无毒产品,符合生物材料的安全性要求。结论海藻酸钙海绵止血作用显著,无明显细胞毒性。
Objective To investigate the hemostatic property of calcium alginate（CA） sponge and its degree of safety on exterior use. Methods The optimal coagulation concentration of CA was determined by Lee-W hite clotting test, and then the CA was freeze-dried to form a sponge. The coagulation effect in vitro of the CA sponge was determined by Blood Clotting Index（BCI） test. The ear artery bleeding model and full-thickness skin wound model of rabbit were employed to determine the hemostatic property of CA sponge. The degree of safety of CA sponge was evaluated by cell toxicity test. Results The BCI was significantly decreased in CA sponge group（33.08±4.02） than that in gelatin sponge（72.05±10.48, P〈0.05）. In rabbit ear artery bleeding test, the volume of blood loss was obviously less in CA sponge group（2.85±0.14g） than that in gelatin sponge group（2.97±0.17g） or hospital gauze group（3.93±0.18 g, P〈0.05）. In rabbit full-thickness skin wounds test, the total amount of blood absorbed was lower in CA sponge group（2.66±0.24） than in hospital gauze group（3.81±0.73, P〈0.05）, but the hemostasis time in CA sponge group（97.83±5.49s） showed no significant difference as compared with that in gelatin sponge group（80.25±7.41 s, P〉0.05）, though they were all shorter significantly than that in hospital gauze group（101.00±14.71 s, P〈0.05）. The cell toxicity of 100% CA sponge was level 1, and it complied with the safety requirements for biomaterials. Conclusion The CA sponge has a good hemostatic property without obvious cytotoxicity.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2016年第3期180-183,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家科技重大专项重大新药创制资助项目(2014ZX09J14103-09C)~~
关键词
海藻酸钙
止血药
明胶海绵
吸收性
细胞毒性
calcium alginate
hemostatics
gelatin sponge
absorbable
cell toxicity
