摘要
目的观察慢传输型便秘型（STC）患者与正常人结肠组织蛋白质表达的差异。方法采用双向凝胶电泳（2-DE）技术，计算机辅助的图像分析以及质谱鉴定方法，对5例STC患者与5例正常人结肠组织蛋白质进行分离和比较分析。结果正常对照组平均胶蛋白斑点数为239个，STC组平均胶蛋白斑点数为215个，与正常对照组平均胶匹配点数为197个，匹配率为82％，有14个蛋白质点的表达量差异有统计学意义（差异倍数〉10，P〈0．05），其中有12个蛋白点表达发生明显上调，有2个蛋白点表达发生明显下调。结论STC患者与正常人结肠组织蛋白质表达谱差异有统计学意义，这些差异蛋白可能参与了STC的发病机制．
Objective To investigate and screen the different expression patterns of proteins be- tween slow transit constipation （STC） group and normal group using a comparative proteomic approach. Methods Two - dimensional eleetrophoresis was applied to separate the proteins in specimens from 5 STC patients and 5 normal controls. The proteins with statistically significant differential expression between two groups were identified by using computer aided image analysis and matrix assisted laser desorption ioniza- tion tandem time of flight mass spectrometry （MALDI- TOF- MS）. Results A total of 239 protein spots were identified in the average gel of the normal controls, and 215 in patients with STC. A total of 197 pro- tein spots were matched and the mean matching rate was 82%. There were 14 protein spots which were ex- pressed with statistically significant differences. Of those 14 protein spots, the expression of 12 spots was increased markedly, while 2 were decreased significantly. Conclusion The proteomics expression in co- lonic specimens of STC patients is statistically significantly different from that of normal contrils, which may be associated with the pathogenesis of STC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第3期607-610,共4页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金（81500505、81570492）
武汉市中青年医学骨干人才项目
湖北省科技计划项目（2015CFB636、2011CDB521）
武汉大学自主科研项目（303410500160）
武汉大学中南医院科研基金项目（22#）
关键词
蛋白质组学
双向凝胶电泳
慢传输型便秘
Proteomics
Two - dimensional electrophoresis
Slow transit constipation
