摘要
以感病的朝天委陵菜（Potentilla supine）叶片为试材,采用病原学及分子生物学方法,研究了河南商丘地区朝天委陵菜白粉病病原菌的系统进化关系。结果表明：该病原菌分生孢子梗直立,圆柱形,简单无分枝,大小为（90~180）μm×（10~17）μm,分生孢子透明,椭圆形,有明显的纤维体,大小为（17~33）μm×（13~18）μm,4~6个串生;病原菌接种叶片病症与自然状态一致,白色菌丝体部分覆盖上层叶表面,随着病菌的发展,菌丝蔓延至覆盖整个叶子表面;对其核糖体DNA内转录间隔区（ITS）序列进行PCR扩增、测序获得563bp序列（GenBank登录号KR049083）。经MEGA 3.1软件分析,其与来自佩兰（Herb eupatorii）棕丝单囊白粉菌（Podosphaere fusca）的序列（GenBank登录号JX548297）聚为一支,而与来自同属另4个种的ITS序列亲缘关系较远。研究表明,河南省商丘地区的朝天委陵菜白粉菌为棕丝单囊白粉菌（Podosphaera fusca）。
Taking infected leaves of Potentilla supine as material,using etiological and molecular systematic identification methods,the phylogenetic evolution relationship of powdery mildew on Potentilla supine was studied in Shangqiu,Henan of China.The results showed that conidiophores of pathogenic fungi were cylindrical,vertical,simple and unbranched,measuring（90-180）μm×（10-17）μm;conidia revealed hyaline,ellipsoid,possessing conspicuous fibrosin bodies measuring（17-33）μm×（13-18）μm,and 4-6conidia formed in chains;pathogenicity of inoculated leaves had no difference with leaves in natural state,symptoms began as white mycelium partially covering upper leaf surfaces;as the disease progressed,it spread to cover entire leaf surfaces;internal transcribed spacer（ITS）sequence of its ribosomal DNA was amplified by PCR and sequenced to obtain the 563 bp target fragment（GenBank accession No.KR049083）.Sequence analysis by MEGA 3.1software revealed that ITS sequence and one sequence from Herb eupatorii,Podosphaera fusca（GenBank accession No.JX548297）gathered together,distantly related to the ITS sequence of other four species in the same genus.The research showed that powdery mildew pathogen of Potentilla supinain Shangqiu was Podosphaera fusca.
出处
《北方园艺》
CAS
北大核心
2016年第6期112-115,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(31571997)
河南省教育厅资助项目(13B210199,15A180019)
关键词
朝天委陵菜
白粉菌
ITS序列
分子系统学鉴定
Potentilla supina
powdery mildew
ITS sequences
molecular systematics identification
