摘要
目的探讨乳腺癌17染色体异倍体（多体、单体）对人表皮生长因子受体-2（HER-2）表达的影响及其临床病理学意义。方法收集2013年1~12月,同时采用免疫组化（IHC）和荧光原位杂交（FISH）技术检测HER-2表达状态的乳腺浸润癌病例,共352例。根据17号染色体多体、单体及HER-2的表达情况分组,对各组患者的临床病理特征进行比较分析。结果 17号染色体多体、单体与HER-2蛋白表达无相关性（P〉0.05）;17号染色体多体与HER-2基因扩增呈负相关（r=-0.162,P=0.001）;17号染色体单体与HER-2基因扩增呈正相关（r=0.282,P=0.000）。17号染色体多体组与HER-2阴性组比较,组织学分级,脉管瘤栓差异有统计学意义（P〈0.05）。17号染色体多体组与HER-2阳性组比较,组织学分级,脉管瘤栓,Ki-67表达,ER、PR阳性表达差异有统计学意义（P〈0.05）。17号染色体单体组与HER-2阴性组比较,PR阳性表达差异有统计学意义（P〈0.05）。17号染色体单体组与HER-2阳性组比较,肿瘤大小差异有统计学意义（P〈0.05）。17号染色体多体组与17号染色体单体组比较,脉管瘤栓,PR阳性表达差异有统计学意义（P〈0.05）。结论 17号染色体多体、单体与HER-2的蛋白表达无相关性,而与HER-2基因扩增有相关性。17号染色体多体组具有独特的临床病理特点,可能提示乳腺癌的预后。
Objective To investigate the impact of chromosome 17heteroploid（polysomy,haploid）of breast cancer on the expression of human epidermal growth factor receptor 2（HER-2）and its clinicopathologic significance.Methods A total of 352 cases of invasive breast cancer were collected from January to December 2013.The expression of HER-2was detected by simultaneously using the fluorescent insituhybridization（FISH）and mimunohistochemistry.The cases were divided into the groups according to the chromosome 17 polysomy,haploid and the expression of HER-2.The clinicopathological features were analyzed and compared among groups.Results The chromosome 17 polysomy and haploid had no correlation with HER-2protein expression（P〉0.05）;chromosome 17 polysomy was negatively correlated with HER-2gene amplification（r=-0.162,P=0.001）;chromosome 17 haploid was positively correlated with HER-2gene amplification（r=0.282,P=0.000）.Compared with the HER-2-negative group,the chromosome 17 polysomy group had a statistically significant difference in histological grade and vascular tumor thrombus（P〈0.05）.Compared with the HER-2-positive group,the chromosome 17 polysomy group had a statistically significant difference in histological grade,vascular tumor thrombus,Ki-67 expression,ER positive expression and PR positive expression（P〈0.05）.Compared with the HER-2-negative group,the chromosome 17 haploid group had a statistically significant difference in PR positive expression（P〈0.05）.The tumor diameter in the chromosome 17 haploid group had a statistically significant difference compared with the HER-2-positive group（P〈0.05）.Compared with the chromosome 17 haploid group,the chromosome 17 polysomy group had a statistically significant difference in vascular tumor thrombus and PR positive expression（P〈0.05）.Conclusion The chromosome 17 polysomy and haploid have no correlation with the HER-2protein expression,but associated with HER-2gene amplification.The chromosome 17 polysomy group has unique clinicopathological features,which may indicate the prognosis in the patients with breast cancer.
出处
《重庆医学》
CAS
北大核心
2016年第9期1228-1232,共5页
Chongqing medicine
