摘要
目的：建立测定痕量格列本脲（GLB）的共振瑞利散射（RRS）光谱法。方法：在pH为3.5的HAC-NaAc缓冲体系中,GLB与曙红Y（EY）形成离子缔合物,会引起RRS光谱的明显改变。结果：最大散射波长位于312nm。散射信号的增强（ΔI_（RRS））与GLB的质量浓度在0.1～2.5mg/mL范围内呈线性关系,检出限（3σ）为25μg/L。方法应用于片剂中GLB的测定,相对标准偏差（n=5）为1.9%。结论：RRS法简便、快速、准确,可用于GLB的定量测定。
Objective：To develop a resonance Reyleigh scattering（RRS）method for the determination of trace Glibenclamid（GLB）.Methods：In pH3.5HAC-NaAc buffer solution,GLB reacted with Eosin Y to form anion-association complexes.As a result,the intensitie of resonance Rayleigh scattering was enhanced greatly and the new scattering spectra displayed.Results：The maximum RRS wavelength at 312 nm.The linear relationship was found between the increases of the scattering intensity（ΔI_（RRS））and the mass concentration of GLB in the range of 0.1~2.5mg/mL with detection limits（3σ）of 25μg/L.The method was applied to determine GLB in tablets,giving the RSD（n=5）of 1.9%.Conclusion：The method is simple,accurate,sensitive and applied to the determination of trace amount of NVB in tablets with satisfactory results.
出处
《长治医学院学报》
2016年第1期13-15,共3页
Journal of Changzhi Medical College
基金
国家级大学生创新创业训练项目(2014299)
长治医学院科技启动基金项目(普及项目)(QDZ201515)
