摘要
目的观测中药配伍组分(40%麦冬多糖、30%黄连生物碱、30%三七总皂苷)对糖尿病血管病变(diabetic angiopathies,DA)模型大兔氧化应激损伤的影响。方法采用四氧嘧啶静脉推注配合腹主动脉内膜球囊损伤术诱导大兔的DA模型,予中药配伍组分高、中、低剂量组及辛伐他汀组干预。结果中药配伍组分对空腹血糖(fasting blood glucose,FBG)、晚期糖基化终末产物(advanced glycation end products,AGEs)明显降低作用,与模型组比较差异有统计学意义(P<0.05),而辛伐他汀组无明显降低FBG、AGEs的作用(P>0.05);此外中药配伍组分及辛伐他汀组对DA模型大兔的一氧化氮(nitrogen oxide,NO)、一氧化氮合酶(nitric oxide synthase,NOS)、丙二醛(malondialdehyde,MAD)均有降低作用,对超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)有升高作用,与模型组比较差异有统计学意义(P<0.05)。结论中药配伍组分对FBG、AGEs、NO、NOS、MDA有抑制作用,可增加SOD、GSH-Px活性,有效地干预DA模型大兔的蛋白非酶糖基化的进程,减轻氧化应激反应造成的损伤。
Objective To explore the effect of traditional Chinese medicine composition（ 40%Ophiopogon japonicus polysaccharide,30% huanglian alkalis,30% Panax notoginseng saponins）（ TCMC） to improve diabetic angiopathies oxidative stress injury in rabbits. Methods DA rabbits were established by alloxan intravenous injection with abdominal aorta intima balloon injury,then intervened by TCMC of high,medium,low dose or simvastatin respectively. Results Compared with model group,TCMC could inhibit the content of fasting blood glucose（ FBG）,advanced glycation end products（ AGEs） in the blood（ P〈0.05） but simvastatin could not（ P〉0. 05）. TCMC and simvastatin could inhibit the content of nitrogen oxide（ NO）,nitric oxide synthase（ NOS）,malondialdehyde（ MDA） but activate superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-PX） in the blood serum（ P〈0. 05）,and there was significant difference compared with model group（ P〈0. 05）. Conclusion TCMC has inhibition on FBG,AGEs,NO,NOS,MDA and could activate SOD,GSH-Px,which leaded to intervention on the process of protein non enzymatic glycosylation of the DA model and improvement on diabetic angiopathies oxidative stress injury.
出处
《环球中医药》
CAS
2016年第3期287-291,共5页
Global Traditional Chinese Medicine
基金
国家自然科学基金(81374026)
第55批中国博士后科学基金(2014M551000)
关键词
中药配伍组分
糖尿病血管病变
氧化应激损伤
机理研究
Traditional Chinese medicine composition
Diabetic angiopathies
Oxidative stress
Mechanism
