摘要
目的研究胃癌组织中MIF与miR-451的表达及临床意义。方法选取60例胃癌患者的肿瘤组织(观察组)及其癌旁组织标本(对照组),采用RT-PCR方法检测胃癌组织和癌旁组织中MIF和miR-451的表达水平,并分析其与临床病理的关系。结果 RT-PCR结果显示,胃癌患者的肿瘤组织标本中MIF表达水平显著高于对照组患者,差异具有统计学意义(P<0.05);miR-451表达水平显著低于对照组患者,差异具有统计学意义(P<0.05)。比较胃癌中MIF与miR-451的表达与临床病理因素关系可知,MIF与miR-451的表达与患者的性别、年龄及远处转移无关(P>0.05),而与肿瘤分化程度、TNM分期、浸润深度、淋巴结转移有关(P<0.05)。经Spearman等级相关分析发现胃癌组MIF与miR-451表达具有显著负相关性(r=-0.697,P=0.012)。结论胃癌组织中MIF的表达显著升高,而miR-451的表达显著下降,且具有明显的相关性,可以作为重要的辅助指标对临床胃癌的诊断、治疗和预后判断进行指导。
Objective To study the expression and clinical significance of miR-451 and MIF in gastric cancer tissues.Methods 60 cases of gastric cancer patients with tumor tissue（observation group）and its adjacent tissue specimens（control group）was selected.The RT-PCR method was used to detect the expression level of miR-451 and MIF in gastric cancer tissues and adjacent tissues.Its relationship with clinical pathology was observed.Results RT-PCR results showed that the expression level of MIF in tumor tissues was significantly higher than that in control group（P〈0.05）.The expression level of miR-451 was significantly lower than that in control group（P〈0.05）.Compared the expression of miR-451 and MIF in gastric cancer,the expression of and MIF was associated with miR-451（P〉0.05）.Which was associated with tumor differentiation,TNM stage,invasion depth and lymph node metastasis（〈0.05P）.Spearman rank correlation analysis showed that the expression of miR-451 and MIF in gastric cancer group had significantly negative correlation（r=-0.697,P=0.012）.Conclusion The expression of MIF in gastric cancer tissues is significantly increased,while the expression of miR-451 was significantly decreased,and it had significant correlation,which can be used as an important assistant index for diagnosis,treatment and prognosis of gastric cancer.
出处
《中国实验诊断学》
2016年第3期375-377,共3页
Chinese Journal of Laboratory Diagnosis
