摘要
目的探讨抗核抗体谱(ANAs)中12项抗体检测结果对诊断系统性红斑狼疮(systemic lupus erythematosus,SLE)的临床应用价值。方法采用线性免疫分析法(line immuno assay LIA)检测86例SLE患者(SLE组)、81例其他风湿性疾病患者(疾病对照组)和90例健康体检者(健康对照组)的血清ANAs中12项抗体,采用SPSS统计学软件对检测结果分析。结果健康对照组中针对12项抗原的抗体未被检出,SLE组和疾病对照组中12项抗体有不同程度的阳性被检出,抗双链DNA抗体(dsDNA)、抗核小体抗体(Nucleosome)、抗核糖核蛋白粒子抗体(Sm)、抗PO抗体(P0)、抗组蛋白抗体(Histone)、抗Ro/SS-A抗体(52 KD)等7项抗体在SLE组的阳性检出率分别为65.12%、53.49%、33.72%、43.02%、47.67%、37.21%、58.14%均明显高于疾病对照组和健康对照组,组间比较差异有统计学意义(P<0.05),疾病对照组与健康对照组间所检测抗体的阳性检出率,比较差异无统计学意义(P>0.05)。SLE组单项抗体检测与多项抗体联检的阳性率,比较差异有统计学意义(P<0.05),疾病对照组单项抗体检测与多项抗体联检的阳性率,比较差异无统计学意义(P>0.05)。结论 ANAs的12项抗体联检有助于提高SLE的阳性检出率,可为疾病的筛查、诊断、分类、病情观察等方面提供有价值的实验室依据,具有较高的临床应用价值。
Objective To investigate the antinuclear antibody（ANAs）in 12 antibody test results for the diagnosis of systemic lupus erythematosus（systemic lupus erythematosus,SLE）value in clinical application.Methods SLE assay（immuno line LIA）was used to testing the serum ANAs of 12patients（SLE group）,81 patients with other rheumatic diseases（control group）and 90 healthy controls（healthy control group）,and the results were analyzed by SPSS software.Results In the healthy control group,12 antibodies were test in SLE group and disease control group.The positive testing rate of anti double stranded SLE antibody（dsDNA）,anti DNA antibody（Nucleosome）,anti PO antibody（P0）,anti KD antibody（Histone）,33.72%,43.02%,47.67%,58.14%,37.21% and 7were significantly higher than those in control group and healthy control group（P〈0.05）,there was no significant difference between the two groups（P〉0.05）in the control group and the healthy control group.The positive rate of SLE group single antibody testing and multiple antibody levels,the difference has statistical significance（P〈0.05）,the disease control group single antibody detection and a number of antibody levels of the positive rate,the difference between no statistical significance（P〉0.05）.Conclusion ANAs 12 antibody levels is helpful to improve the SLE were test,the positive rate of disease screening,diagnosis,classification,condition observation provides valuable laboratory basis and has a high value in clinical application.
出处
《中国实验诊断学》
2016年第3期422-424,共3页
Chinese Journal of Laboratory Diagnosis
关键词
系统性红斑狼疮
抗核抗体谱
线性免疫分析法
风湿性疾病
Systemic Lupus Erythematosus
Anti nuclear antibody spectrum
linear immuno assay
Rheumatic diseases
