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持续被动运动对兔骨关节炎软骨细胞中Akt活性及细胞凋亡的影响 被引量:7

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摘要 目的观察持续被动运动(CPM)对兔骨关节炎(OA)软骨细胞Akt活化情况及凋亡情况的影响。方法 36只新西兰大白兔随机分为3组,每组12只:A、对照组,B、模型组,C、治疗组。B组和C组采用Huulth方法进行造模,C组兔子在术后第1天即开始进行持续被动运动(CPM)治疗,8 h/d,连续8周,8周后处死所有兔子。观察关节软骨形态并进行苏木精—伊红染色法(HE)染色观察软骨组织形态。流式细胞术检测关节软骨细胞凋亡情况。Western blot方法检测关节软骨细胞Akt磷酸化活化情况。结果同对照组相比,治疗组兔膝关节软骨大体评分情况明显改善,同时软骨组织形态也出现明显的改善。流式细胞染色结果显示,与模型组软骨细胞凋亡率(39.85±9.93)%相比,治疗组软骨细胞凋亡率(11.98±4.38)%显著降低(P<0.01)。Western blot结果显示,对照组软骨细胞Akt活化水平为(6.85±1.16)%,模型组软骨细胞Akt活化水平为(1.46±0.37)%,治疗组软骨细胞Akt活化水平为(5.95±1.48)%,与模型组相比,治疗组软骨细胞Akt活化水平显著升高(P<0.01)。结论 CPM可通过影响兔OA膝关节软骨细胞的Akt通路活化水平,进而降低软骨细胞的凋亡情况,从而改善OA病情。
出处 《中国临床研究》 CAS 2016年第3期381-383,共3页 Chinese Journal of Clinical Research
基金 新疆维吾尔自治区自然科学基金资助项目(2013211A060)
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参考文献14

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同被引文献164

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  • 2洪俊雄.老年人练太极拳应慎防膝关节损伤[J].当代体育科技,2011,1(3):81-82. 被引量:3
  • 3王国金,卫小春,张志强,纪斌平,毕树雄,刘巧玲.血清和关节液中基质金属蛋白酶-3及其抑制因子-1的检测及临床意义[J].中华风湿病学杂志,2006,10(5):311-313. 被引量:20
  • 4尚平,贺宪,陈孝银,才忠民,刘志祥,项旭军,唐勇.过伸位和过屈位固定复制骨性关节炎动物模型的比较[J].生物骨科材料与临床研究,2006,3(1):11-14. 被引量:32
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  • 6Osajie FE, Yakubu K.A retrospective non-comparative analysis of the quality of care for osteoarthritis at the general out-patient department of Jos University Teaching Hospital, Nigeria.J Family Med Prim Care. 2015; 4(2):217-220.
  • 7Tsolis KC, Bei ES, Papathanasiou I, et al. Comparative proteomic analysis of hypertrophic chondrocytes in osteoarthritis. Clin Proteomics.2015; 12(1):12.
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  • 10Kitay GS, Koren M J, Helfet DL,et al.Efficacy of combined local mechanical vibrations, continuous passive motion and thermotherapy in the management of osteoarthritis of the knee. Osteoarthritis Cartilage. 2009; 17(10): 1269-1274.

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