摘要
目的:建立高效液相色谱同时测定不同产地、不同品种绞股蓝药材中芦丁和槲皮素含量的方法。方法:ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5μm),流动相乙腈(A)-0.2%磷酸溶液(B)梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长360 nm,进样量10μL。结果:芦丁在2.535~10.14μg线性良好(R^2=0.999 2),平均回收率为98.72%,RSD 1.2%;槲皮素在0.018 1~0.144 8μg线性良好(R^2=0.999 3),平均回收率98.40%,RSD 1.7%。结论:该方法简便可行、重复性好,适用于绞股蓝药材2种有效成分的同时测定,可为绞股蓝药材的质量控制提供参考依据。
Objective： To establish a determination method for different varieties of rutinand quercetin in Gynostemma pentaphyllum from different producing areas. Method： HPLC was performed on ZORBAX SB-C18column（ 4. 6 mm × 150 mm,5 μm）,eluting in a gradient mode with the mobile phase of acetonitrile（ A）-0. 2%H3PO4as（ B） at a flow rate of 1. 0 m L·min- 1. The column temperature was 30 ℃. The detection wavelength was360 nm. The injection volume was 10 μL. Result： The linearity of rutin was good within the range of 2. 535-10. 14 μg（ R^2= 0. 999 2）,and the average recovery was 98. 72%（ RSD 1. 2%）. The linearity of quercetin was good within the range of 0. 018 1-0. 144 8 μg（ R^2= 0. 999 3）,and the average recovery of 98. 40%（ RSD 1. 7%）.Conclusion： The established method is simple,feasible and highly reproducible,and can be used for determining rutin and quercetin in G. pentaphyllum and providing reference for quality control of G. pentaphyllum.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第6期45-47,共3页
Chinese Journal of Experimental Traditional Medical Formulae
