摘要
目的:观察不同剂型七味白术散对肠道菌群失调模型小鼠小肠黏膜上皮白细胞介素-4(IL-4),IL-10,干扰素-α(IFN-α)mRNA表达的影响。方法:采用盐酸林可霉素和头孢曲松钠注射液联合中药番泻叶ig小鼠制备肠道菌群失调腹泻模型。造模成功后,将小鼠随机分为模型组(MG)(随后称自然恢复组ZHG),七味白术散水煎液组(QJG,6 g·kg-1),七味白术散滴剂组(QDG,1.36 g·kg-1),七味白术散提取物组(QTG,0.27 g·kg-1),并设正常组(NG)。MG(ZHG)与NG给予蒸馏水ig,其余各治疗组小鼠给予相应剂型药物ig。在造模第7,9,11,13天,各组各时间点取10只小鼠小肠行逆转录-聚合酶链式反应(RT-PCR)检测小鼠小肠黏膜上皮IL-4,IL-10,IFN-αmRNA的表达。结果:在不同时间点,QJG,QTG和QDG的IL-4,IL-10mRNA表达均高于MG(P<0.05)。MG的IFN-αmRNA表达明显高于NG,QJG,QTG,QDG(P<0.05);在不同时间点,QJG,QTG,QDG小鼠肠道黏膜上皮IFN-αmRNA表达逐渐下降(P<0.05)。结论:不同剂型七味白术散均可能通过上调肠道黏膜上皮释放抑炎因子IL-4,IL-10和下调IFN-α的表达,减轻肠道炎症,促进受损黏膜组织修复,但七味白术散滴剂和七味白术散提取物调节免疫功能要优于七味白术散水煎剂。
Objective： To investigate the effects of different formulated Qiwei Baizhu San on the mRNA expression of interleukin-4（ IL-4）,IL-10 and interferon-α（ IFN-α） in intestinal mucosal epithelia of mice with intestinal flora alteration. Method： Ceftriaxone,lincomycin hydrochloride and folium sennae were administered to mice by gavage to establish intestinal flora alteration type diarrhea models. After modeling,the mice were randomly divided into model group（ MG,referred to as natural recovery group ZHG）,Qiwei Baizhu decoction group（ QJG,6 g·kg- 1）,Qiwei Baizhu drops group（ QDG,1. 36 g·kg- 1）,Qiwei Baizhu extract group（ QTG,0. 27 g·kg- 1）,and normal group（ NG）. Mice in MG（ ZFG） and NG were given with distilled water by ig administration,and the mice in rest treatment groups were given with appropriate dosage of the drugs by ig administration. At days 7,9,11 and 13 of modeling,intestine was taken from 10 mice in each group for RT-PCR analysis of IL-4,IL-10 and IFN-αmRNA in intestinal mucosal epithelia of the mice. Result： Expressions of IL-4 mRNA and IL-10 mRNA in QJG QTG and QDG were higher than those in MG（ P 〈0. 05） at different treatment time. Expressions of IFN-α mRNA in NG,QJG,QTG and QDG were significantly lower than those in MG（ P〈 0. 05）. At the different treatment time,IFN-α mRNA expression in intestinal mucosal epithelia of mice in QJG,QTG and QDG was gradually decreased（ P 〈0. 05）. Conclusion： Different formulations of Qiwei Baizhu San can attenuate the intestinal inflammation,promote repair of damaged mucosa tissues by up-regulating the expressions of IL-4 and IL-10,and down-regulating the expression of IFN-α in intestinal mucosal epithelia. In addition,Qiwei Baizhu drops and Qiwei Baizhu extract are superior to Qiwei Baizhu decoction in regulating immune functions.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第6期84-88,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81273990)
国家教育部博士导师基金项目(2013000011)
湖南省中医药管理局重点项目(1300001)
关键词
七味白术散
剂型
菌群失调
免疫调节
细胞因子
Qiwei Baizhu San
formulation type
intestinal flora alteration
immunoregulation
cytokine
