摘要
目的观察骨化三醇对博莱霉素诱导的大鼠肺纤维化的影响,初步探讨骨化三醇抗肺纤维化的机制。方法将大鼠随机分为对照组（CO组）、模型组（MO组）、地塞米松组（DE组）和骨化三醇组（VD组）。MO组、DE组、VD组经气管内注入博莱霉素（5mg/kg）制备小鼠肺纤维化模型,对照组大鼠同法注射等体积生理盐水。于造模当天开始每天给药,DE组及VD组分别给予地塞米松4mg/kg、骨化三醇胶丸0.5μg/kg灌胃,CO组及MO组给予等体积蓖麻油灌胃。在造模后14、28d分别处死各组部分小鼠,取肺组织进行苏木精-伊红染色、MASSON染色观察,应用免疫组化方法测定肺组织中转化生长因子β1（TGF-β1）、Ⅲ型胶原的含量。结果肺组织病理学检查结果符合肺纤维化的典型表现,提示造模成功。与CO组比较,MO组大鼠气道和肺组织出现慢性炎症改变和结构破坏,VD组及DE组上述病理变化较MO组有明显改善。MO组大鼠肺组织TGF-β1、Ⅲ型胶原的表达较CO组显著升高,VD组及DE组TGF-β1、Ⅲ型胶原的表达显著低于MO组（F=8.578~33.868,P〈0.05）。结论骨化三醇可减轻博莱霉素诱导的肺纤维化,其作用机制可能与降低TGF-β1及Ⅲ型胶原的表达有关。
Objective To investigate the effect of ealcitriol on pulmonary fibrosis （PF） in rat model induced by bleomycin and its intervention mechanism. Methods Forty SD male rats were equally randomized to four groups as control group （CO）, model group （MO）, dexamethasone-treated group （DE） and calcitriol-treated group （VD）. A model of PF was created in MO, DE and VD groups by intratracheal injection of bleomycin （5 mg/kg）, for those in the control group, equal-volume normal saline was intratracheally injected. Starting from the day on completion of modelling, the rats in DE group and VD group were given intragas- trie administration of dexamethasone （4 mg/kg） and calcitriol （0.5 μg/kg）, respectively, daily~ those in the CO and MO groups were given intragastric administration of equal-volume castoroil. After creation of the models, the rats in each group were sacri- ficed, respectively, on days 14 and 28, to take lung tissue for HE and Masson staining, the contents of transforming growth factor- β1（TGF-β1） and collagen-Ⅲ in lung tissue were detected using histoimmunochemical method. Results The findings of patholo- gy were consistent with typical manifestations of fibrosis of lung, demonstrating success of modelling. Compared with the control group, the lung tissue of rats in the model group showed chronic inflammation and disorganization, the above pathological changes in VD and DE groups markedly improved. In model group, the expressions of TGF-β1 and collagen-Ⅲ significantly increased, and that in the VD group and DE group decreased （F=8.578-33.868,P〈0.05）. Conclusion Calcitriol can reduce bleomycin-in- duced pulmonary fibrosis, its mechanism may be associated with decreasing expressions of TGF-β1 and collagenⅢ .
出处
《青岛大学医学院学报》
CAS
2016年第1期57-60,共4页
Acta Academiae Medicinae Qingdao Universitatis
关键词
骨化三醇
博莱霉素
肺纤维化
转化生长因子Β1
胶原Ⅲ型
calcitriol
blemycin
pulmonary fibrosis
transforming growth factor beta1
collagen type Ⅲ
