摘要
目的建立银杏叶提取物中总黄酮醇苷的HPLC含量测定方法。方法采用Hypersil ODS2色谱柱（4.6×250 mm,5μm）,以甲醇-0.4%磷酸溶液（55：45）为流动相,检测波长360 nm,流速1.0 ml/min,柱温35℃。结果槲皮素、山奈酚、异鼠李素进样量分别在10.0-60.0μg/ml、10.0-60.0μg/ml、1.0-30.0μg/ml范围内线性关系良好,平均回收率分别为99.42%、99.92%、98.11%。结论本方法简单、准确,可以用于银杏叶提取物中总黄酮醇苷的定量分析。
Objective： To establish an HPLC method for the determination of the contents of total flavone glycosides in Ginkgo biloba extract. Methods： The analyses were performed on a Hypersil ODS2 column（ 4. 6 × 250 mm,5 μm）,and the mobile phase was methanol-water containing 0. 4% phosphoric acid（ 55： 45） at the flow rate of 1 ml / min. The detection was carried out at 360 nm, and the column temperature was 35℃. Results： The calibration curves of quercetin,kaempferide and isorhamnetin had good linearinities with good recoveries in the ranges of 10. 0 - 60. 0μg / ml,10. 0 -60. 0μg / ml and 1. 0 - 30. 0μg / ml,respectively. The average recoveries of the method were 99. 42%,99. 92% and98. 11% respectively. Conclusion： The HPLC method is simple,accurate,which can be used for the determination of the contents of total flavone glycosides in Ginkgo biloba extract.
出处
《泰山医学院学报》
CAS
2016年第1期33-35,共3页
Journal of Taishan Medical College
基金
国家级大学生创新创业训练计划项目(201410439028)
