摘要
目的对检测水中大肠菌群的方法进行对比分析,为实际检测工作中更好地选择检测方法提供参考。方法采用51孔定量盘法、97孔定量盘法、乳糖蛋白胨发酵法和乳糖胆盐发酵法对水样中的大肠菌群进行检测。结果检测生活饮用水131份(出厂水79份、末梢水52份),酶底物法的阳性检出率73.3%,乳糖蛋白胨发酵法的阳性检出率61.8%,乳糖胆盐发酵法的阳性检出率58.8%。结论 51孔定量盘法灵敏度比多管发酵法好,但定量范围窄;97孔定量盘法灵敏度、定量范围均好于多管发酵法。
Objective To compare two methods in detecting comliform in water for choosing preferable detection method. Methods 51-hole quantitative plate method,97-hole quantitative plate method,lactose peptone fermentation and lactose bile salts fermentation were all applied for determining the coliform in water. Results There were 131 samples of drinking water detected（ 79 of finished water and 52 of tap water）,and the positive rate was 73. 3%,61. 8% and 58. 8% with enzyme substrate technique,lactose peptone fermentation and lactose bile salts fermentation,respectively. Conclusion The sensitivity of 51- hole quantitative plate is preferable to that of multiple tube fermentation and 97-hole quantitative plate is preferable to multiple tube fermentation in terms of sensitivity and quantitative range.
出处
《预防医学情报杂志》
CAS
2016年第3期254-257,共4页
Journal of Preventive Medicine Information
关键词
大肠菌群
酶底物法
乳糖蛋白胨发酵法
乳糖胆盐发酵法
coliform
enzyme substrate
lactose peptone fermentation
lactose bile salts fermentation
