摘要
目的通过线性探针方法与传统药敏试验(比例法)在四川省自贡市涂阳肺结核患者中耐药检测相比,评价线性探针方法用于检测临床耐药肺结核患者的可行性。方法收集自贡市涂阳肺结核患者痰标本以及涂阳患者阳性培养物,以传统比例法药敏试验为标准获得探针检测异烟肼(INH)、利福平(RFP)耐药的灵敏度与特异度。结果线性探针检测92份涂阳肺结核患者痰标本异烟肼耐药检测的灵敏度与特异度分别为78.6%、98.8%;利福平耐药检测的灵敏度与特异度分别为88.2%、94.5%,耐多药检测的灵敏度与特异度分别为83.3%、97.4%;202株菌株中异烟肼耐药检测的灵敏度与特异度分别为80.6%、98.8%,利福平耐药检测的灵敏度与特异度分别为82.9%、98.1%,耐多药检测的灵敏度与特异度分别为77.3%、98.9%。结论线性探针方法适用于临床涂阳肺结核患者异烟肼、利福平耐药快速检测。
Objective By comparing with the traditional drug sensitive testing（ scaling method） for drug resistance test on clinical patients with smear positive pulmonary tuberculosis in Zigong to evaluate the feasibility of line probe assay for detecting clinical patients with drug-resistant tuberculosis. Methods Sputa samples and culture content of smear-positive patients were collected. The sensitivity and specificity of line probe assay on Isoniazid and Rifa mpicin with the lowenstein-jensen culture and scaling method as the standard methods. Results The sensitivity and specificity of Line probe assay on 92 sputa samples fro m clinical smear-positive pulmonary tuberculosis patients were respectively 78. 6% and 98. 1% for the Isoniazid resistance test,88. 2% and 94. 5%for Rifampicin resistance test and 83. 3% and 97. 4% for multidrug resistance test. The sensitivity and specificity of Line probe assay on 202 bacteria were respectively 80. 6% and 98. 8% for Isoniazid,82. 9% and 98. 1% for Rifampicin,and 77. 3% and 98. 9% for multidrug resistance test. Conclusion Line probe assay can be used to rapid test of Isoniazid,Rifampicin and multidrug resistance test for patients with clinical s mear-positive pul monary tuberculosis.
出处
《预防医学情报杂志》
CAS
2016年第3期285-288,共4页
Journal of Preventive Medicine Information
关键词
结核病
耐多药
线性探针
tuberculosis
multidrug resistance
line probe assay
