摘要
A novel copper（Ⅱ） complex based on chiral amino-alcohol derived Schiff base ligand,[Cu_4（R-L）_4（H_2O）_2]·（CH_3COOH）_2·（H_2O）（1,（R）-H_2 L =（R）-3-phenyl-2-（2-hydroxy-3-methoxybenzylideneamino） propane-1-ol）,was synthesized and characterized by EA,IR,UV-Vis,ESI-MS,circular dichroism spectra and single-crystal X-ray diffraction.Complex 1 crystallizes in orthorhombic,space group Ρ2_12_12 with a = 15.7660（14）,b = 49.526（3）,c = 10.4213（9） A,V = 8137.2（12） A^3,Ζ = 4,C_（72）H_（81）Cu_4N_4O_（19）,Mr = 1560.57,μ = 1.096 mm^-1,F（000） = 3244,Flack = 0.06（3）,the final R = 0.0924 and w R = 0.2451（I 〉 2σ（I）） for 41108 observed reflections.The interactions of the complex with calf thymus DNA（CT-DNA） were investigated by some spectroscopic technique methods.The results show the complex exhibits strong binding with CT-DNA.In addition,in vitro cytotoxicity test of 1 towards four kinds of human cancerous cell lines（He La,HL-60,Caco-2 and A549） showed substantial cytotoxic activity.The experimental investigations indicated that the chirality of complex 1 play an important role in cytotoxicity and interactions with DNA.
A novel copper（Ⅱ） complex based on chiral amino-alcohol derived Schiff base ligand,[Cu_4（R-L）_4（H_2O）_2]·（CH_3COOH）_2·（H_2O）（1,（R）-H_2 L =（R）-3-phenyl-2-（2-hydroxy-3-methoxybenzylideneamino） propane-1-ol）,was synthesized and characterized by EA,IR,UV-Vis,ESI-MS,circular dichroism spectra and single-crystal X-ray diffraction.Complex 1 crystallizes in orthorhombic,space group Ρ2_12_12 with a = 15.7660（14）,b = 49.526（3）,c = 10.4213（9） A,V = 8137.2（12） A^3,Ζ = 4,C_（72）H_（81）Cu_4N_4O_（19）,Mr = 1560.57,μ = 1.096 mm^-1,F（000） = 3244,Flack = 0.06（3）,the final R = 0.0924 and w R = 0.2451（I 〉 2σ（I）） for 41108 observed reflections.The interactions of the complex with calf thymus DNA（CT-DNA） were investigated by some spectroscopic technique methods.The results show the complex exhibits strong binding with CT-DNA.In addition,in vitro cytotoxicity test of 1 towards four kinds of human cancerous cell lines（He La,HL-60,Caco-2 and A549） showed substantial cytotoxic activity.The experimental investigations indicated that the chirality of complex 1 play an important role in cytotoxicity and interactions with DNA.
基金
supported by the Natural Science Foundation of Shandong Province(No.ZR2013BM017)
the Natural Science Foundation of China(No.21105042)
