摘要
为了明确引起河南省大蒜病毒病的病原,采用RT-PCR方法对采集的表现矮缩症状的大蒜样品分别用5对引物进行检测,结果表明,在大蒜样品中同时检测到洋葱黄矮病毒（onion yellow dwarf virus,OYDV）、韭葱黄条病毒（leek yellow stripe virus,LYSV）和青葱潜隐病毒（shallot latent virus,SLV）3种病毒,没有检测到大蒜普通潜隐病毒（garlic common latent virus,Gar CLV）和青葱X病毒（allexiviruses）。根据测序结果进行序列和遗传进化分析发现,河南省OYDV分离物与SLV分离物在进化树上都单独形成一分支,其中OYDV与其他分离物的核苷酸同源性为94.8%~96.9%,SLV与其他分离物的核苷酸同源性在86.5%~88.5%,河南省LYSV分离物与墨西哥分离物的同源性达到99.4%,亲缘关系最近。试验中同时检测到3种病毒,说明河南省大蒜病毒病为多种病毒复合侵染。
To determine the pathogen causing garlic dwarf,garlic samples showing dwarf symptom were collected for RT-PCR analysis using five primer pairs. The results showed that onion yellow dwarf virus（ OYDV）,leek yellow stripe virus（ LYSV） and shallot latent virus（ SLV） were detected in the sample,while garlic common latent virus（ Gar CLV） and shallot X virus（ allexiviruses） were not found.According to the sequence and genetic evolution analysis,OYDV and SLV Henan isolates both belonged to a separate branch with the nucleotide homology with other isolates between 94. 8% and 96. 9% for OYDV and between 86. 5% and 88. 5% for SLV. LYSV Henan isolate was closely related to Mexio isolate with the homology of 99. 4%. This test simultaneously detected three kinds of viruses,indicating that garlic virus disease in Henan was induced by complex infection of a variety of viruses.
出处
《河南农业科学》
CSCD
北大核心
2016年第3期102-105,共4页
Journal of Henan Agricultural Sciences
基金
河南省高等学校青年骨干教师资助计划项目(2014GGJS-037)
关键词
大蒜病毒病
分子检测
序列分析
河南省
garlic virus disease
molecular detection
sequence analysis
Henan province
