摘要
为研究猪源乙脑病毒分离株基因遗传稳定性,对猪源乙脑病毒分离株BSF.ZZ-1和BSF.ZZ-3在BHK-21细胞上连续传代60次后的病毒全基因组进行序列测定及分析。结果表明,BSF.ZZ-1-p60、BSF.ZZ-3-p60基因组全长均与各自的亲本毒株BSF.ZZ-1、BSF.ZZ-3一致,均为10 977 nt,在2个病毒基因组的10 701 nt位点处均插入1个碱基G。与亲本毒株相比,2个细胞传代毒株的病毒基因组经连续传代后趋于稳定,均有10个位点发生氨基酸突变,这些突变主要集中在E蛋白区域。核苷酸序列同源性比对分析发现,与其他JEV参考毒株相比,BSF.ZZ-1-p60和BSF.ZZ-3-p60与弱毒疫苗株SA14-14-2的核苷酸序列同源性较高,分别为99.6%和95.9%,其氨基酸序列与猪源野毒分离株SH0601和HW的氨基酸序列同源性较高,分别为98.2%、86.4%和98.3%、86.5%。
In this study,the full-length genomes of two Japanese encephalitis virus（ JEV） strains,BSF.ZZ-1-p60 and BSF. ZZ-3-p60 that were isolated from pigs and passaged on BHK-21 Cells,were sequenced and compared with reference strains. The results showed that same to the parental viruses,both of the genomes of BSF. ZZ-1-p60 and BSF. ZZ-3-p60 were 10 977 nt long,with an insert base of G at the 10 701 nt site. Compared to the parental strains,the viral genomes of BSF. ZZ-1-p60 and BSF. ZZ-3-p60 were stabilized after passaged on BHK-21 cells for 60 times,with a total of 10 amino acid mutations which we mainly occurred in E proteins in both strains. Compared to the reference JEV strains,both BSF. ZZ-1-p60 and BSF. ZZ-3-p60 showed highest nucleotide sequence homologies（ 99. 6% and 95. 9%） to vaccine strain SA14-14-2,while for the amino acid sequences,both strains showed highest homologies（ 98. 2%,86. 4% and 98. 3%,86. 5%） to that of the pig-originated strains SH0601 and HW,respectively.
出处
《河南农业科学》
CSCD
北大核心
2016年第3期135-140,共6页
Journal of Henan Agricultural Sciences
基金
国家公益性行业(农业)科研专项(201203082-5)
河南省科技攻关计划项目(152102110127)
河南省农业技术推广财政补助资金项目(YCN201519111)
河南省农科院科研发展专项资金项目(20157817)
