摘要
为明确黄淮麦区TaGS2等位基因的分布状况及其与主要农艺性状的关系,对黄淮麦区2008年之前育成的种质材料、新育成的品种(系)及国外引进材料,用TaGS2-A1、TaGS2-B1和TaGS2-D1等功能标记鉴定对应的基因,并结合相关农艺性状发掘优势单倍型。结果表明,黄淮麦区2008年之前育成的种质材料和新育成品种(系)中TaGS2等位基因分布频率存在一定的差异;TaGS2-A1b、TaGS2-B1b和TaGS2-D1a是优势TaGS2等位基因,TaGS2-A1b在小麦抽穗期、株高和小穗数的改良上是优势单倍型,但在新育成的品种(系)中有下降的趋势,TaGS2-D1a能够显著增加小穗数、穗粒数和穗粒重,在各类材料中的比例都较高;TaGS2-B1b是提高千粒重的优势单倍型。因此,在黄淮麦区小麦穗部性状改良中TaGS2-B1b和TaGS2-D1a的作用显著,尤其是TaGS2-D1a,同时黄淮麦区种植小麦遗传多样性在减少,一些优势单倍型未受到重视,应对地方品种和一些国外引进材料加以利用。
Glutamine synthetase(GS)plays a key role in the nitrogen(N)use and yield potential of bread wheat.Investigating the haplotypes in TaGS2 genes and their associations with agronomic traits may be a useful approach for improving wheat yield and N-use efficiency.In this study,278 elite wheat varieties from Huang-Huai winter wheat region and 57 introduced varieties were used to discover the frequencies of TaGS2 haplotypes and the association between TaGS2 and agronomic traits,in addition,corresponding allelic variants were also identified using the function markers of TaGS2 genes.The results indicated that the frequency of allelic variation of TaGS2 genes detected were different between those wheat varieties,three favorable TaGS2 haplotypes(TaGS2-A1 b,TaGS2-B1 band TaGS2-D1a)were revealed,but the frequencies of TaGS2-A1 band TaGS2-B1 bamong varieties in different years presented a downward trend.TaGS2-A1 b was considered as a potential haplotype for improving heading date,plant height and spikelet number per ear,TaGS2-D1 a was associated with significantly higher values in spikelet number per ear,grain number per ear and grain weight per ear,while TaGS2-B1 b was a potential superior haplotype for increasing 1 000-grain weight,nevertheless,TaGS2-D1 afor improving spike traits may be more valued by many breeders.In the Huang-Huai wheat region,the genetic diversity of wheat varieties was reducing,so we should pay more attention to landrace and some introduced varieties.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2016年第3期281-286,共6页
Journal of Triticeae Crops
基金
国家重点基础研究计划项目(2014CB138100)
陕西省自然科学基金项目(2015JM3094)
陕西省重点科技创新团队资助项目(2014KCT-25)