摘要
目的:研究米非司酮对子宫内膜异位症(Ems)模型大鼠炎症因子表达的影响。方法:取SD大鼠,随机分为正常(生理盐水)组、模型(生理盐水)组和米非司酮低、中、高剂量[0.65、1.30、2.60 mg/(kg·d)]组,每组10只。除正常组外,其余各组大鼠复制Ems模型,各组大鼠ig相应剂量的药物,连续4周。比较各组大鼠治疗前后异位病灶体积,酶联免疫吸附法检测各组大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6、IL-8含量,Western blot法分析各组大鼠异位病灶组织中环氧化酶2(COX-2)、前列腺素E2(PGE_2)蛋白表达及核因子κB(NF-κB)p65磷酸化水平,实时定量-聚合酶链反应法检测各组大鼠异位病灶组织中NF-κB p65m RNA表达水平。结果:与正常组比较,模型组大鼠异位病灶体积和血清TNF-α、IL-6、IL-8含量增加,病灶组织中COX-2、PGE_2蛋白和NF-κB p65磷酸化水平及m RNA表达增强(P<0.01);与模型组比较,米非司酮各剂量组大鼠异位病灶体积减小,血清TNF-α、IL-6、IL-8含量降低,病灶组织中COX-2、PGE_2和NF-κB p65磷酸化水平及m RNA表达减弱(P<0.01),且呈剂量依赖性。结论:米非司酮能减小Ems异位病灶体积,可能与抑制NF-κB信号通路和降低炎症因子表达有关。
OBJECTIVE：To study the effects of mifepristone on the expression of inflammatory factors in endometriosis（Ems）model rats. METHODS：SD rats were randomly divided into normal group（normal saline）,model group（normal saline）,mifepristone low-dose,medium-dose and high-dose groups [0.65,1.30,2.60 mg/（kg·d）],with 10 rats in each group. Except for normal group,Ems model was induced in other groups,and they were given relevant medicine intragastrically for consecutive 4weeks. The volume of the ectopic focus was compared before and after treatment. The serum contents of TNF-α,IL-6 and IL-8were detected by ELISA method. The phosphorylation of NF-κB p65 and the expression of COX-2 and PGE_2 were detected by Western blot. The expression of NF-κ B p65 m RNA in ectopic focus was detected by RT-PCR. RESULTS：Compared with normal group,the volume of the ectopic focus,the serum contents of TNF-α,IL-6 and IL-8,the expression of COX-2 and PGE_2 protein,the phosphorylation of NF-κB p65 and the expression of NF-κB p65 m RNA in ectopic focus were increased in model group（P0.01）. Compared with model group,the volume of the ectopic focus,the serum contents of TNF-α,IL-6 and IL-8,the expression of COX-2 and PGE_2 protein,the phosphorylation of NF-κB p65 and the expression of NF-κB p65 m RNA in ectopic focus were decreased in mifepristone groups（P0.01）,in dose-dependent manner. CONCLUSIONS：Mifepristone can reduce the volume of the Ems ectopic focus,via blocking NF-κB signaling pathway and reducing the expression of inflammatory factors.
出处
《中国药房》
CAS
北大核心
2016年第10期1374-1377,共4页
China Pharmacy
