摘要
目的探讨梗阻性黄疸患者梗阻解除前后血清补体C5a水平的变化情况及其与肝纤维化标志物的相关性。方法收集2012年6月-2014年6月在本院行经内镜逆行胰胆管造影的梗阻性黄疸患者164例,选取20例健康人作为对照组。检测血清补体C5a水平和Ⅳ型胶原、Ⅲ型前胶原、层黏连蛋白、透明质酸4项肝纤维化标志物水平。组间比较采用t检验,相关性分析采用线性相关分析。结果梗阻性黄疸患者血清补体C5a水平较健康对照人群升高[(89.7±30.2)vs(62.2±21.1)ng/L,t=2.213,P=0.016),并与梗阻病程密切相关(r=0.954,P=0.003)。解除梗阻后血清补体C5a水平降至(66.2±26.3)ng/L,与解除前相比差异有统计学意义(t=1.998,P=0.021)。解除梗阻前与血清补体C5a水平呈同步升高相关性的有Ⅳ型胶原(r=0.976,P<0.001)、Ⅲ型前胶原(r=0.972,P<0.001)、层黏连蛋白(r=0.915,P=0.039)和透明质酸(r=0.962,P=0.002);解除梗阻后与血清补体C5a水平呈同步下降相关性的有Ⅳ型胶原(r=0.965,P=0.001)、Ⅲ型前胶原(r=0.912,P=0.003)和透明质酸(r=0.875,P=0.023)。结论补体C5a可能参与梗阻性黄疸所致肝纤维化的发生过程。
Objective To investigate the change in the serum level of complement C5 a after obstruction removal and its correlation with the serum levels of liver fibrosis markers in patients with obstructive jaundice. Methods A total of 164 patients with obstructive jaundice who underwent endoscopic retrograde cholangiopancreatography and obstruction removal in our hospital from June 2012 to June 2014 were enrolled,and 20 healthy adults were enrolled as the control group. The serum levels of complement C5 a and four liver fibrosis markers,type Ⅳcollagen（ ⅣP）,pre- type Ⅲ collagen（ PⅢP）,laminin（ LN）,and hyaluronic acid（ HA）,were measured. The t- test was applied for comparison between groups,and linear correlation analysis was applied for correlation analysis. Results Compared with the healthy controls,the patients with obstructive jaundice had a significantly higher serum level of complement C5a（ 89. 7 ± 30. 2 vs 62. 2 ± 21. 1 ng / L;t = 2. 213,P = 0. 016）,and the serum level of complement C5 a was closely related to the course of obstruction（ r = 0. 954,P = 0. 003）.The serum level of complement C5 a decreased significantly after obstruction removal（ 66. 2 ± 26. 3 ng / L; t = 1. 998,P = 0. 021）. Before obstruction removal,the serum level of complement C5 a increased synchronously with those of ⅣP（ r = 0. 976,P 〈 0. 001）,PⅢP（ r =0. 972,P 〈 0. 001）,LN（ r = 0. 915,P = 0. 039）,and HA（ r = 0. 962,P = 0. 002）; after obstruction removal,the serum level of complement C5 a decreased synchronously with those of ⅣP（ r = 0. 965,P = 0. 001）,PⅢP（ r = 0. 912,P = 0. 003）,and HA（ r = 0. 875,P =0. 023）. Conclusion Complement C5 a may be involved in the development of liver fibrosis induced by obstructive jaundice.
出处
《临床肝胆病杂志》
CAS
2016年第3期495-498,共4页
Journal of Clinical Hepatology
基金
国家自然科学基金资助项目(30971355)
关键词
肝硬化
黄疸
阻塞性
补体C5A
liver cirrhosis
jaundice,obstructive
complement C5a
