摘要
目的建立高效液相色谱法（HPLC法）测定生化汤协定方代煎液中游离阿魏酸和总阿魏酸含量的方法。方法应用HPLC法测定游离阿魏酸和总阿魏酸的含量,以Diamonsil C18柱（4.6 mm×250mm,5μm）为色谱柱,甲醇-1%醋酸溶液（30∶70）为流动相,检测波长为321 nm。结果游离阿魏酸在进样量0.212～1.060μg范围内呈良好的线性关系,回归方程为Y=4.685 5×106X-2.109 0×104（r=0.999 9）,游离阿魏酸平均加样回收率100.60%,相对标准偏差（RSD）=2.33%,总阿魏酸平均加样回收率99.43%,RSD=2.65%。结论本方法操作简单,方法可靠,结果准确,灵敏度高,重现性好。
Objective To establish a method for determination of free and total ferulic acid in decoction of Shenghua prescription by high performance liquid chromatography（ HPLC）.Methods On Diamonsil C18（ 4.6mm × 250 mm,5 μm）; with methyl alcohol（ containing 1 % acetic acid） = 30∶ 70 as mobile phase and UV determination at 321 nm.Results A good linearity was obtained over the range of 0.212 ～ 1.060 μg for free ferulic acid,regression equation was Y = 4.685 5 × 106X-2.109 0 × 104（ r = 0.999 9）.The average recoveries of free and total ferulic acid components were 100.60% with relative standard deviation（ RSD） = 2.33% and 99.43% with RSD = 2.65%,respectively.Conclusion This method is simple,reliable,accuracy,with high sensitivity and better repeatability.
出处
《河北中医》
2016年第2期265-267,277,共4页
Hebei Journal of Traditional Chinese Medicine
关键词
色谱法
高压液相
生化汤
阿魏酸
Chromatography
High pressure liquid
Shenghua decoction
Ferulic acid
