摘要
目的探讨牡荆苷对小鼠脑缺血/再灌注损伤后脑组织中丙二醛(malondialdehyde,MDA)、一氧化氮(nitric oxide,NO)含量和超氧化物歧化酶(superoxide dismutase,SOD)、一氧化氮合酶(nitric oxide synthase,NOS)活性的影响。方法雄性昆明种小鼠70只,随机分为正常对照组、假手术组、模型组、尼莫地平组(0.6mg·kg^(-1))、牡荆苷组(10、20、40 mg·kg^(-1))。采用线栓法制备小鼠脑缺血/再灌注损伤模型,采用试剂盒法测定MDA、NO含量和SOD、NOS活性。结果再灌注24h后,小鼠脑缺血/再灌注后神经功能评分模型组显著升高(P<0.01),牡荆苷组显著降低(P<0.05)。模型组脑组织中MDA、NO含量及NOS活性显著升高(P<0.01),SOD活性显著降低(P<0.01);牡荆苷组脑组织中MDA、NO含量和NOS活性显著降低(P<0.05),SOD活性增强(P<0.01)。结论牡荆苷脑保护作用可能是通过抗氧化应激作用实现的。
Objective To investigate the effects of vitexin on the content of MDA and NO and the activities of SOD and NOS in mice with cerebral ischemia-reperfusion injury.Methods 70 Kunming male mice were randomly divided into the control group,sham-operation group,cerebral ischemia-reperfusion group,nimodipine group（0.6mg·kg^-1）and vitexin groups（10,20,40 mg·kg^-1）.Mice were prepared with cerebral ischemia-reperfusion injury by suture method,and then the contents of MDA and NO and the activities of SOD and NOS were determined by kit method.Results After reperfusion 24 h,neurological deficit score of cerebral ischemia-reperfusion group was significantly higher than that of the shamoperation group（P〈0.01）;whereas those of vitexin groups were significantly lower than the value of the model group（P0.05）.The contents of MDA and NO and the activity of NOS significantly increased（P0.01）,the activity of SOD significantly decreased（P0.01）in cerebral ischemia-reperfusion group,vitexin groups significantly reduced the contents of MDA and NO and the activity of NOS（P0.05）,enhancing the activity of SOD（P0.01）.Conclusion The protective effect of vitexin on cerebral ischemia-reperfusion injury might be produced by anti-oxidative stress.
出处
《河北北方学院学报（自然科学版）》
2016年第2期19-22,共4页
Journal of Hebei North University:Natural Science Edition
基金
河北省卫生厅项目(No.20150474)
