摘要
利用13个源自转录组序列的SNP标记对3个马氏珠母贝(Pinctada fucata)家系进行遗传多态性分析及聚类分析。结果显示,家系1#、3#、6#的平均期望杂合度(He)分别为0.307 8、0.318 9和0.382 7;平均观测杂合度(Ho)分别为0.342 2、0.341 0和0.394 2;平均多态信息含量(PIC)分别为0.243 5、0.247 9和0.297 7。结果表明,3个马氏珠母贝家系具低度或中度遗传多态性,为马氏珠母贝内SNP应用于遗传多态性分析等提供了基础。利用SNP标记对3个家系进行聚类分析,发现半同胞家系1#和3#在家系及个体聚类中均先聚在一起,然后再与亲缘关系较远的家系6#聚在一起。新开发的SNP标记能较准确地对家系及个体进行聚类,为SNP标记应用于马氏珠母贝遗传关系分析提供了研究基础。
A transcription database was tracked to identify and validate SNP markers. A total of thirteen SNPs were used for genetic analysis in Pinctada fucata. For Pinctada fucata family 1^#, 3^# and 6^#, the average expected heterozygosities（ He）were 0.307 8, 0.318 9 and 0.382 7, respectively; the average observed heterozygosities（Ho） were 0.342 2, 0.341 0 and0.394 2, respectively; and the average polymorphism information contents（ PIC） were 0.243 5, 0.247 9 and 0.297 7,respectively. The half sibling family 1#and 3#were firstly clustered together in the dendrogram, and then clustered with family6#, which was in accordance with the genetic relationship. The development of novel SNPs in Pinctada fucata can provide genetic tools for genetic diversity analysis and clustering analysis.
出处
《海洋通报》
CAS
CSCD
北大核心
2016年第1期96-102,共7页
Marine Science Bulletin
基金
国家高技术研究发展计划(863计划)(2012AA10A410)
广东省海洋渔业科技推广专项(A201301A03)
广东省科技计划(2013B020308005)
关键词
马氏珠母贝
SNP
遗传多态性
聚类分析
Pinctada fucata
single nucleotide polymorphism
genetic diversity
cluster analysis
