摘要
背景:骨髓间充质干细胞良好的增殖分化能力和旁分泌功能使其在肠道疾病方面具有不可替代的优势。目的:探讨骨髓间充质干细胞移植对大鼠小肠缺血再灌注损伤的影响。方法:取48只SD大鼠建立小肠缺血再灌注损伤模型,随机分为2组:在实验组肠系膜下注射1 m L骨髓间充质干细胞,对照组肠系膜下注射等量的生理盐水,于移植后0,2,6,24,72,120 h留取大鼠血清,ELISA法检测血清中二胺氧化酶、肿瘤坏死因子α、D-乳酸水平;移植后24 h取小肠组织光镜下观察病理变化,透射电镜下观察紧密连接变化,免疫组化法检测ZO-1蛋白表达。结果与结论:1骨髓间充质干细胞移植后6 h和24 h时实验组血清二胺氧化酶、肿瘤坏死因子α和D-乳酸水平低于对照组(P<0.05)。2骨髓间充质干细胞移植组小肠细胞坏死、绒毛水肿、肠道充血及炎细胞浸润较对照组轻。3骨髓间充质干细胞组ZO-1蛋白表达高于对照组。4结果表明小肠缺血再灌注损伤大鼠肠黏膜下移植骨髓间充质干细胞可以改善小肠黏膜的通透性。
BACKGROUND： Bone marrow mesenchymal stem cells have good proliferation and paracrine functions, which have irreplaceable advantages in the treatment of intestinal diseases. OBJECTIVE： To explore the effects of bone marrow mesenchymal stem cell transplantation on intestinal ischemia-reperfusion injury in rats. METHODS： Forty-eight Sprague-Dawley rats were enrolled to make animal models of ischemic reperfusion injury of the intestine, and then model rats were randomized into experimental and control groups. After modeling, 1 mL bone marrow mesenchymal stem cells or the same volume of normal saline were injected into the intestinal mucosa of rats in the two groups, respectively. At hours 0, 2, 6, 24, 72, 120 after injection, serum diamine oxidase, tumor necrosis factor α, and D-lactic acid levels were detected by ELISA method. At 24 hours after injection, rat intestinal tissues were taken and observed pathologically under light microscopy, and their close connections were observed under transmission electron microscope. ZO-1 protein levels were detected by immunohistochemistry method. RESULTS AND CONCLUSION： Compared with the control group, the serum diamine oxidase, tumor necrosis factor α, and D-lactic acid levels were significantly lower in the experimental group at hours 6 and 24 after injection（P〈0.05）. Intestinal necrosis, villous edema, intestinal congestion and inflammatory cell infiltration in the experimental group were milder than those in the control group. In addition, the ZO-1 protein expression in the experimental group was higher than that in the control group. Experimental results show that bone marrow mesenchymal stem cell transplantation into the intestinal mucosa can improve the intestinal mucosal permeability in rats with intestinal ischemia-reperfusion injury.
出处
《中国组织工程研究》
CAS
北大核心
2016年第6期861-867,共7页
Chinese Journal of Tissue Engineering Research
基金
河南省科技厅基础科技攻关课题(122300450037)~~
