摘要
目的分离鉴定安徽地区野生铁皮石斛内生真菌,旨在丰富其内生真菌资源种类。方法通过组织块法对内生真菌进行分离,运用形态学和分子生物学方法进行鉴定。结果从中分离得到11株内生真菌,鉴定为5属11种。结论铁皮石斛内生真菌种类资源广泛分布,为研究其与宿主石斛共生关系提供理论依据。
Objective To isolate and identify the endophytic fungi from Anhui wild Dendrobium officinale to enrich the types of re-sources in the endophytic fungi.Methods The endophytic fungi were isolated from root,stem and leaf of the cultivated Dendrobium officinale by tissue inoculation culture,respectively,and then were identified by morphology and rDNA sequence analysis.Results 11 strains from the cultivated Dendrobium officinale were identified as 11 species and 5 genera.Conclusions Endophytic fungi exist richly in Dendrobium officinale,which provides theoretical basis for the research on the symbiotic relationship between the Dendrobium officinale and endophytic fungi.
出处
《安徽医药》
CAS
2016年第3期429-432,共4页
Anhui Medical and Pharmaceutical Journal
基金
国家中医药管理局“中药化学”重点学科建设(No 2012-2017)
关键词
铁皮石斛
内生真菌
分离
鉴定
Dendrobium officinale
endophytic fungi
isolation
identification
