摘要
目的:采用DNA甲基化芯片技术探讨高脂饮食对Apo E-/-小鼠动脉粥样硬化模型全基因组DNA甲基化的影响。方法:30只雄性Apo E-/-小鼠随机分为正常组与高脂组,每组15只,正常组给予正常饲料喂养,高脂组给予高脂饲料喂养。16周后,测其血脂、血清同型半胱氨酸水平(Hcy)水平、血清DNA甲基化与血清DNA甲基化转移酶(DNMTs)水平;采用DNA甲基化芯片检测两组小鼠主动脉组织全基因组甲基化情况。结果:与正常组相比,高脂组小鼠血清CHOL、TG、LDL-C均显著升高,HDL-C显著下降;血清DNA甲基化水平与血清DNA甲基化转移酶(DNMTs)水平均显著升高。甲基化芯片结果显示:与正常组相比,高脂组主动脉全基因组中共有875个基因甲基化发生改变,差异具有统计学意义(P<0.05),其中高甲基化基因数目496,占总数56.69%;低甲基化基因数目379,占总数的43.31%。结论:高脂饲料可升高主动脉基因组甲基化水平,降低基因组的表达,可能是Apo E-/-小鼠容易形成动脉粥样硬化的机制之一。
To investigate the effects of high-fat diet on genome DNA methylation in Apo E-/-mice model of atherosclerosis by biochip technology. Methods： 30 male Apo E-/-mice were divided into normal group and high-fat group with 15 in either group. Normal group were fed with normal diet while the high-fat group were given high fat diet.16 weeks later, the blood lipids,and the levels of homocysteine（Hcy）, DNA methylation and DNA methylation transferase（DNMTs） in serum were measured; DNA methylation chip was used to detect the genome-wide methylation in the aorta of two groups. Results： Compared with the normal group,CHOL, TG and LDL- C of the high-fat group of mice were significantly increased（P〈0.01）, and HDL- C was decreased significantly（P〈0.05）. The levels of DNA methylation and DNA methylation transferase（DNMTs） were significantly increased as well（P〈0.05）.According to the methylation chip, a total number of 875 genes in high-fat group were changed on DNA methylation in contrast with the normal group（P〈0.05）, of which 496 were hypermethylation, 56.69 % of the total, and 379 were hypomethylation, 43.31 % of the total.Conclusions： The high-fat diet can increase the level of aortic genome methylation that inhibits the genes expression which might be one of the mechanisms of Apo E-/- mice easily to form atherosclerosis.
出处
《现代生物医学进展》
CAS
2016年第8期1415-1419,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81303086)
北京市自然科学基金项目(7142037)
