摘要
目的:研究EB病毒(EBV)感染患儿SH2D1A mRNA表达情况,探讨SH2D1A mRNA表达改变与疾病的关系及其意义。方法:选取2014年10月至2015年2月湖南省儿童医院肝病中心收治的初诊为EB病毒感染的患儿,选取同期年龄、性别与EB病毒感染组匹配的健康体检儿童为对照组。检测EB病毒感染组和对照组的SH2D1A mRNA表达水平,计算基因相对表达量,检测血生化指标。结果:EB病毒感染组共32例,其中普通传染性单核细胞增多症(普通组)20例,重症传染性单核细胞增多症(重症组)9例,慢性活动性EBV感染(慢性组)1例,EBV相关噬血淋巴组织细胞增多症(噬血组)2例;对照组10例。各组与对照组SH2D1A mRNA的相对中位表达量分别为25.78、40.14、38.72、18.27、4.65(H=10.68,P<0.05),EB病毒感染组患儿的SH2D1A mRNA表达水平显著高于对照组,重症组、慢性组SH2D1A mRNA表达水平较普通组、噬血组升高(P<0.05),但重症组与慢性组比较差异无统计学意义(P>0.05)。SH2D1A mRNA表达水平与初诊时最低的纤维蛋白原、CD4+、CD4/+CD8+水平呈负相关(P均<0.05),与血清铁蛋白、丙氨酸氨基转移酶、乳酸脱氢酶、甘油三酯、EBV-DNA水平无相关性。结论:EBV感染患儿外周血细胞SH2D1A mRNA表达水平显著升高,造成机体细胞免疫功能紊乱。
Objective： To research the expressions of SH2D1 A mRNA in children with EB virus（ EBV） infection,to discuss the relationship and significance between the change of SH2D1 A mRNA expression and the disease. Methods： Children who were diagnosed as EB infection were selected as EB virus infection group from October 2014 to February 2015 in Liver Diseases Center of Children＇s Hospital of Hunan Province. We selected healthy children who were at the same age,sex as the control group. The levels of SH2D1 A mRNA in EB virus infection group and control group were detected,the relative expression quantities of genes were calculated,and the blood biochemical indexes were tested. Results： The total cases of EB virus infection group was 32,including the common infectious mononucleosis（ group common） 20 cases,severe infectious mononucleosis（ group severity） 9 cases,chronic active EBV infection（ group chronicity） 1 cases,EBV associated hemophagocytic lymphohistiocytosis（ group HLH） 2 cases. The control group was 10 cases.The SH2D1 A mRNA relative expression median levels in each group and the control group were 25.78,40.14,38.72,18.27,4.65（ H= 10.68,P〈0.05）. The SH2D1 A mRNA levels of EB virus infection group were significantly higher than those of control group. The SH2D1 A mRNA levels in group severity,group chronicity increased significantly than those of group common,group HLH,but there were no statistical significance between group severity and group chronicity. The expression level of SH2D1 A mRNA was negatively correlated with the initial fibrin minimum raw and CD4＋,CD4＋/ CD8＋levels（ P〈0.05）,and it had no correlation with serum ferritin,alanine aminotransferase, lactate dehydrogenase, triglyceride, EBV-DNA levels. Conclusion： The levels of SH2D1 A mRNA in peripheral blood cells of EBV infection were markedly increase. EB virus infection can result in cellular immune function disorder.
出处
《儿科药学杂志》
CAS
2016年第4期1-4,共4页
Journal of Pediatric Pharmacy
基金
2012年湖南省卫生厅科研基金指导课题
项目编号C2012-016
