摘要
目的建立穿王消炎片中瑞香素含量测定的方法。方法采用Agilent Eclipse SB-C18柱（250mm×4.6mm,5μm）色谱柱,以甲醇-0.1%磷酸（40∶60）为流动相,检测波长345nm,流速1.0ml.min-1,柱温35℃。结果果瑞香素在4.9325~49.3250μg.ml-1浓度范围内线性关系良好,r=0.9994,平均回收率为99.03%,RSD为1.76%。结论论方法简便、结果准确,可用于穿王消炎片的质量控制。
Objective To establish an method for determining the content of daphnetin in Chuan Wangxiaoyan pills. Methods The HPLC system consisted of the Agilent SB-C18（250mm×4.6mm,5μm） and methanol- 0.1% phosphoric acid（40∶60） as the mobile phase,The flow rate was 1.0m L·min-1 and the detectivewavelength was 345 nm. The flow rate was 1.0ml.min-1 and the column temperature was 35 ℃. Results There was good linearity ofdaphnetin within the range of 4.9325~49.3250μg/ml（r=0.9994）.The average recovery was 99.03%, and RSD was 1.76%（n=9）.Conclusion The method is simple and accurate. It is suitable for the quality control of Chuan Wangxiaoyan pills.
出处
《首都食品与医药》
2016年第8期84-85,共2页
Capital Food Medicine
