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肌苷对CPZ介导的急性脱髓鞘小鼠行为学及皮质髓鞘的影响 被引量:2

Effect of inosine on behavior and cortex myelin in CPZ mouse model of acute demyelination
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摘要 目的:探讨肌苷对双环己酮草酰二腙(cuprizone,CPZ)介导的急性脱髓鞘小鼠行为学及皮质髓鞘的影响。方法:在普通饲料中掺入0.2%CPZ,饲养小鼠6 w,同时联合腹腔注射肌苷,制备脱髓鞘治疗模型,利用体重测量、Morris水迷宫、悬尾实验、透射电镜等技术,观察肌苷对脱髓鞘小鼠治疗后行为学及皮质髓鞘的影响。结果:(1)体重变化:与生理盐水对照组比较,CPZ损伤组、肌苷治疗组小鼠体重从第6 d开始均明显降低(P<0.05);(2)行为学:Morris水迷宫空间定位实验中,与CPZ损伤组比较,肌苷治疗组平台象限停留时间明显增加(P<0.05);悬尾实验中,小鼠6 min不动时间均无明显差异(P>0.05);(3)皮质髓鞘电镜观察显示,肌苷治疗组小鼠的皮质髓鞘病理改变程度降低,有新生髓鞘。结论:通过含0.2%CPZ饲料饲养联合腹腔注射肌苷制备脱髓鞘治疗小鼠,可使小鼠的学习、记忆功能明显改善,皮质髓鞘有明显的修复与再生,揭示肌苷对伴有学习记忆功能障碍的脱髓鞘小鼠有髓鞘保护作用。 Objective: To explore the effect of inosine on behavior and cortex myelin in cuprizone(CPZ) mouse model of acute demylination. Methods: C57 BL /6 mice was fed with standard diet that was blended with 0. 2% CPZ,and inosine was injected intraperitoneally to cure acute demyelination for 6 weeks. Morris water maze test,tail suspension test and transmission electron microscope( TEM) were used to observe learning and memory function and cortex myelin of ice. Results:(1) Weight changes: Weight gain of the CPZ injury group and the inosine treatment group significantly less than that of the saline control group from the sixth day( P〈 0. 05),( 2) Behavioral changes: Morris water maze test showed that escape latency in navigation test of the inosine treatment group was significantly longer than that of the CPZ injury group( P〈 0. 05), The tail suspension test showed that the immobility time was no significant differences in the groups.(3) TEM observation : Compared with the CPZ injury group,the degree of abnormalities structural of myelin was reduced,and there were a lot of new myelin sheaths. Conclusion: Simultaneous administration of inosine effectively attenuated cuprizone induced demyelination,and impoved the learning and memory function,and the remyelination of cortex was obvious. These results suggest that inosine exerts protective effect on demyelination in CPZ mouse model.
出处 《神经解剖学杂志》 CAS CSCD 北大核心 2016年第2期201-204,共4页 Chinese Journal of Neuroanatomy
基金 国家自然科学基金(81460214) 宁夏自然科学基金(NZ14058)
关键词 肌苷 CPZ 脱髓鞘 精神分裂症 小鼠 ionsine CPZ demyelination schizophrenia mouse
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参考文献15

  • 1John MG,Sukanta S,David C,et al.Schizophrenia:a concise overview of incidence,prevalence,and mortality[J].Epidemiol Rev,2008,30:67-76.
  • 2Xiao XL,Dawson N,Mac Intyre L,et al.Exploring metabolic pathway disruption in the subchronic phencyclidine model of schizophrenia with the Generalized Singular Value Decomposition[J].BMC Syst Bio,2011,5:72-92.
  • 3曹江.精神分裂症的治疗策略进展[J].医学理论与实践,2015,28(17):2297-2299. 被引量:8
  • 4Rimol LM,Nesvag R,Hagler DJ,et al.Cortical volume,surface area,and thickness in schizophrenia and bipolar disorder[J].Biol Psychiatry,2012,71:552-560.
  • 5Silvestroff L,Bartucci S,Pasquini J,et al.Cuprizone-induced demyelination in the rat cerebral cortex and thyroid hormone effects on cortical remyelination[J].Exp Neurol,2012,235:357-367.
  • 6Chandran P,Upadhyay J,Markosyan S,et al.Magnetic resonance imaging and histological evidence for the blockade of cuprizone-induced demyelination in C57BL/6 mice[J]Neuroscience,2012,202:446-453.
  • 7Yan G,Xuan YH,Dai ZZ,et al.Brain metabolite changes in subcortical regions after exposure to cuprizone for 6 weeks:potential implications for schizophrenia[J].Neurochem Res,2015,40:49-58.
  • 8Kovacs Z,Kekesi KA,Doboly A,et al.Absence epileptic activity changing effects of non-adenosine nuckeoside inosine,guanosine,guanosine and uridine in Wistar Albino Glaxo Rijswijk rats[J].Neuroscience,2015,300:593-608.
  • 9姚庆和,高国栋.肌苷对帕金森病小鼠模型的神经保护作用[J].第四军医大学学报,2005,26(2):141-145. 被引量:20
  • 10康庆云,杨理明,陈波,张洁,郭峰.肌苷、维生素B_6联合羚羊角颗粒治疗儿童抽动障碍的对照研究[J].药学与临床研究,2013,21(5):563-565. 被引量:4

二级参考文献62

  • 1李华.大剂量肌苷、VitB_6联合耳穴治疗儿童抽动障碍[J].社区医学杂志,2005,3(8):12-13. 被引量:2
  • 2钟佑泉,吴惧,谢晓丽,胡文广,周文智.耶鲁抽动症整体严重度量表对抽动障碍患儿的临床评估[J].中国实用儿科杂志,2006,21(3):214-216. 被引量:126
  • 3Kawai H, Makino Y, Hirobe M, et al. Novel endogenous 1,2,3,4-tetrahydroisoquinoline derivatives: Uptake by dopamine transporter and activity to induce parkinsonism [J]. Neurochem, 1998;70:745-751.
  • 4Donnan GA, Willis GL, Kaczmarczyk SJ, et al.Motor function in the 1-methyl-4-pheny-1,2,3,6-tetrahydropyridine-treated mouse [J].J Neurol Sci, 1987;77(2-3):185-191.
  • 5Litsky ML, Hohl CM, Lucas JH, et al. Inosine and guanosine preserve neuronal and glial cell viability in mouse spinal cord cultures during chemical hypoxia[J]. Brain Res ,1999; 821:426-432.
  • 6Shi M, You SW, Meng JH, et al. Direct protection of inosine on PC12 cells against zinc-induced injury[J].Neuroreport , 2002;13(4):477-479.
  • 7Benowitz LI, Jing Y, Tabibiazar R, et al. Axon outgrowth is regulated by an intracellular purine-sensitive mechanism in retinal ganglion cells[J].J Biol Chem,1998; 273(45):29626-29634.
  • 8Benowitz LI, Goldberg DE, Irwin N. A purine-sensitive mechanism regulates the molecular program for axon growth[J].Restor Neurol Neurosci ,2001;19 (1-2):41-49.
  • 9Benowitz LI, Goldberg DE, Madsen JR, et al. Inosine stimulates extensive axon collateral growth in the rat corticospinal tract after injury[J].Proc Natl Acad Sci ,1999;96:13486-13490.
  • 10Peng Chen, David E, Goldberg DE,et al. Inosine induces axonal rewiring and improves behavioral outcome after stroke [J].Proc Natl Acad Sci, 2002;99 (13 ):9031-9036.

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